ABSTRACT
This study was aimed to investigate the effect of T cells activated by DCs loaded with whole antigens of U266 cells on the U266 cells survival in vitro. Peripheral blood mononuclear cells were isolated from healthy donor, and adherented on culture plate. Adherent cells were cultured in AIM-V serum-free medium or in RPMI 1640 medium contained 20% fetal bovine serum (FBS), supplemented with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). Methyl thiazolyl tetrazolium (MTT) assay was used to evaluate killing rate of U266 cells by T cells activated by DCs loaded with whole antigen of U266 cells. The results showed that DCs derived from peripheral blood mononuclear cells cultured by AIM-V serum-free medium or RPMI 1640 medium containing FBS had similar immunophenotype. T cells activated by DCs loaded with whole antigen of U266 cells or mature DCs might kill U266 cells in a dose-dependent manner. It is concluded, DCs derived from peripheral blood mononuclear cells of healthy donor and loaded with whole antigen of U266 cells can induce anti-myeloma response of T cells in vitro.