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PURPOSE@#COVID-19 is also referred to as a typical viral septic pulmonary infection by 2019-nCoV. However, little is known regarding its characteristics in terms of systemic inflammation and organ injury, especially compared with classical bacterial sepsis. This article aims to investigate the clinical characteristics and prognosis between COVID-19-associated sepsis and classic bacterial-induced sepsis.@*METHODS@#In this retrospective cohort study, septic patients with COVID-19 in the intensive care unit (ICU) of a government-designed therapy center in Shenzhen, China between January 14, 2020 and March 10, 2020, and septic patients induced by carbapenem-resistant klebsiella pneumonia (CrKP) admitted to the ICU of the Second People's Hospital of Shenzhen, China between January 1, 2014 and October 30, 2019 were enrolled. Demographic and clinical parameters including comorbidities, critical illness scores, treatment, and laboratory data, as well as prognosis were compared between the two groups. Risk factors for mortality and survival rate were analyzed using multivariable logistic regression and survival curve, respectively.@*RESULTS@#A total of 107 patients with COVID-19 and 63 patients with CrKP were enrolled. A direct comparison between the two groups demonstrated more serious degrees of primary lung injury following 2019-nCoV infection (indicated by lower PaO@*CONCLUSION@#Critical COVID-19 shares clinical characteristics with classical bacterial sepsis, but the degree of systemic inflammatory response, secondary organ damage and mortality rate are less severe. However, following 2019-nCoV infection, the level of immunosuppression may be increased and thus induce in more death at the later stage of patients' hospitalstay.
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Humans , COVID-19 , Carbapenems , Hospital Mortality , Intensive Care Units , Klebsiella , Prognosis , Retrospective Studies , SARS-CoV-2 , SepsisABSTRACT
IDH mutation is prevalent in lower-grade glioma and secondary glioblastoma. Patients bearing IDH mutation are char-acterized by overproduction of 2-HG. 2-HG plays a role in regu-lation of DNA and histone hypermethylation in glioma, thus re-sulting in impaired cell differentiation and tumor formation. As a surrogate marker of mutant IDH, there is increasing interest in development of detection methods for 2-HG. LC-MS is widely used in detecting 2-HG in vitro, and reliable measurement of 2-HG by the non-invasive MRS has been tested in vivo and ex vivo previously. However, whether 2-HG could represent an inde-pendent predictor of patient survival or other clinical features for glioma still needs further study. In this review, we summarize the mechanism adopted by 2-HG in glioma initiation and pro-gression, as well as the detection method tested in clinic. We try to provide guidance to the future combination therapy using mu-tant IDH inhibitors.
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AIM To establish an HPLC method for the simultaneous content determination of seven constituents in Gnaphalium affine D.Don.METHODS The analysis of 80％ methanol of G.affine was performed on a 30 ℃ Atlantis (C) T3 column (4.6 mm× 250 mm,5 μm),with the mobile phase comprising of acetonitrile-formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 288 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (R2 ≥0.999 8),whose average recoveries were 98.58％-103.8％ with the RSDs of 0.88％-1.74％.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of G.affine.
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Objective Aleutian disease, mink enteritis and canine distemper are the three major diseases affecting health of mink. This study intends to establish a multiplex PCR assay for simultaneously detecting of these three viruses. Methods According to the conservative sequences reported in GenBank, three pairs of specific primers were designed to amplify the DNA templates of Aleutian mink disease parvovirus (ADV), mink enteritis parvovirus (MEV), and RNA templates of canine distemper virus (CDV), and optimized the amplifying conditions. Results The specific objective strips of 601 bp ( ADV) , 205 bp ( MEV) and 451 bp ( CDV) were amplified simultaneously. The sensitivity test showed that the lowest nucleic acid detection limits were 2. 67 × 104 copies perμL for ADV, 3. 02 × 104 copies perμL for MEV, and 1. 72 × 105 copies perμL for CDV. The results of test of the clinical samples showed that the multiple PCR and single PCR assay were consistent. Conclusions The established multiplex PCR assay in this study can be used to rapidly detect the clinical samples of ADV, MEV and CDV single or mixed infections.
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In China, many surveys have shown that most people do not have a correct understanding about cold and administration of anti-cold Chinese patent medicine preparations. The author conducted a systematic summary and analysis on the actual application of anti-cold Chinese patent medicine preparations as well as the warning on safe application of anti-cold Chinese patent medicine preparations in Clinical Medication Information of China Pharmacopoeia, in the expectation of reducing the blind application of anti-cold Chinese patent medicine preparations and providing traditional Chinese medicine pharmacists new ideas in monitoring the safe application of exterior syndrome-relieving Chinese patent medicine preparations.
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Humans , China , Common Cold , Drug Therapy , Drugs, Chinese Herbal , Chemistry , Therapeutic Uses , Nonprescription Drugs , Chemistry , Therapeutic UsesABSTRACT
<p><b>OBJECTIVE</b>To evaluate the therapeutic effect of compound anisodine (CA) for patients with primary open angle glaucoma (POAG).</p><p><b>METHODS</b>According to the modified Hodapp-Parrish-Anderson Visual Fields Grading System, 46 patients with moderate stage POAG were randomized to receive compound anisodine injection (CA group) or venoruton tablets (control group). Visual acuity (VA), IOP, fundus, visual fields (VF) and the blood flow of optic nerve were observed.</p><p><b>RESULTS</b>The mean of defect (MD) was decreased in CA group after treatment. The PSV and EDV of ophthalmic artery were remarkably improved in both groups, as well as the PSV, EDV and RI of retinal central artery. Compound anisodine was superior in improving hemodynamics of ophthalmic artery and retinal central artery to venoruton.</p><p><b>CONCLUSION</b>Compound anisodine can protect optic nerve of POAG through improving the visual function and blood supply of optic nerve.</p>
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Adult , Female , Humans , Male , Middle Aged , Glaucoma, Open-Angle , Drug Therapy , Scopolamine Derivatives , Therapeutic Uses , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To evaluate the efficacy of porous polyethylene channel implants in reconstruction of orbital blowout fractures.</p><p><b>METHODS</b>Twenty-three patients with orbital blowout fractures were admitted in our eye center from June 2003 to January 2006. Porous polyethylene channel implants were used to repair the defects of orbital wall and to correct the enophthalmos. The visual acuity, the degree of enophthalmos, diplopia and position were recorded preoperatively and postoperatively.</p><p><b>RESULT</b>No patients presented loss or apparent descent of visual acuity after surgery. Postoperative mean enophthalmos (1.7+/-0.5)mm was smaller significantly than that of preoperation [(3.9 +/-1.2)mm (P<0.05)]. Diplopia disappeared in 10 patients 1 month after surgery. Diplopia disappeared in 11 patients in primary position, but it remained in other positions. Diplopia remained in primary position in 2 patients. Ocular movement in 22 patients was normal postoperatively. While primary position upward occurred in 1 patient postoperatively and 2 weeks later it became normal.</p><p><b>CONCLUSION</b>Porous polyethylene channel implants are effective in reconstruction of orbital blowout fractures.</p>
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Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Young Adult , Orbital Fractures , General Surgery , Polyethylene , Porosity , Prostheses and Implants , Plastic Surgery Procedures , Treatment OutcomeABSTRACT
A strain of Cellulosimicrobium cellulans Ha8 was studied on its morphological, biological characteristics and its utilization of several kinds of benzoic compounds, the results showed this strain was Gram-positive, the long rod-shaped cells were changed into short rod-shape gradually. pH value from pH 6.0 to pH 9.0 and the temperature from 20 ℃ to 40 ℃ were good for its growth. It could not only hydrolyze protein and starch, use cellulose and pectin, decomposite chitin, liquify gelatin and fix nitrogen, but also use phenol, xylene, benzoic, cinnamic acids and diphenlamine as the sole carbon resource for its growth. It could tolerate 0 mmol/L~30 mmol/L, 0 mmol/L~8 mmol/L, 0 mmol/L~30 mmol/L, 0 mmol/L~15 mmol/L and 0 mmol/L ~ 40 mmol/L of benzoic acids, phenol, xylene, cinnamic acids and diphenlamine seperately, but could not use 2,4-dinitrophenol, o-Nitrophenol, 2-Methoxyphenol, aminobenzenesulfonic acid, catechol and o-Phenanthroline as its sole carbon resource.
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<p><b>OBJECTIVE</b>This study is aimed at describing the clinical outcome of amniotic membrane transplantation for exposure of porous sphere implants.</p><p><b>METHODS</b>A retrospective review of consecutive cases of porous sphere orbital implant exposure was carried out. Eight cases were presented between May 2004 and Oct. 2006 (5 males, 3 females; mean age 44.5 years). Six had enucleation and two had evisceration. Exposure occurred in two primary and six secondary. Orbital implant diameter was 22 mm in seven cases and 20 mm in one case. Six patients are with hydroxyapatite and two with high-density porous polyethylene (Medpor) orbital implants. The mean time from implantation to exposure was 1.1 months (range 0.8-2 months). All patients required surgical intervention.</p><p><b>RESULTS</b>The time of follow-up ranged from 3.0 to 28.0 months (mean 16.5 months). Amniotic membrane grafting successfully closed the defect without re-exposure in all of these patients. The grafts were left bare with a mean time to conjunctiva of about 1 month (range 0.8-1.5 months).</p><p><b>CONCLUSION</b>Exposed porous sphere implants were treated successfully with amniotic membrane graft in all of patients. The graft is easy to harvest. This technique is useful, dose not lead to prolonged socket inflammation and infection, and it is valuable application extensively.</p>
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Adult , Female , Humans , Male , Middle Aged , Amnion , Transplantation , Graft Survival , Ophthalmologic Surgical Procedures , Methods , Orbital Implants , Prosthesis Implantation , Methods , Retrospective Studies , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To introduce the clinical effect among patients who received an unwrapped orbital implant with high density porous polyethylene material (Medpor) after enucleation or evisceration.</p><p><b>METHODS</b>Retrospective analysis of a series of 302 patients with anophthalmia who underwent placement of an unwrapped high density porous polyethylene orbital implant. We compared the patients (n=180) who accepted primary implant placement with those (n=122) who accepted secondary implant placement. Parameters evaluated included: age at time of surgery, date of surgery, sex, implant type and size, surgery type, the surgical procedure and technique performed, and complications.</p><p><b>RESULTS</b>The time of follow-up ranged from 2.0 to 58.0 months (mean 32.5 months). A total of 5 of 302 (1.66%) cases had documented postoperative complications. The following problems were noted after surgery: implant exposure, 3 patients (0.99%); implant removed due to orbital infection, 1 patient (0.34%); ptosis, 1 patient (0.34%). There were no significant complications observed in other 297 cases and all implants showed good orbital motility. The clinical effect of primary implant placement is better than that of secondary placement.</p><p><b>CONCLUSION</b>High density porous polyethylene material can be used successfully as an unwrapped orbital implant in anopthalmic socket surgery with minimal complications. The material is well tolerated, nonantigenic and has low rate of infection and migration.</p>
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Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Biocompatible Materials , Follow-Up Studies , Orbital Implants , Polyethylenes , Prosthesis Implantation , Retrospective StudiesABSTRACT
<p><b>OBJECTIVE</b>To evaluate the postoperative complications, the appearance and motility in patients who underwent placement of Medpor orbital implant after enucleation, or secondary implantion.</p><p><b>METHODS</b>A retrospective study was carried out on the 266 cases in whom a Medpor orbital implant was implanted from 2001 to 2004.</p><p><b>RESULTS</b>All the cases were followed up from 3 to 26 months. A total of 23 in 266 (8.6%) cases had documented postoperative complications: inflammatory granuloma in 2 cases (0.8%), and moderate or severe ptosis in 4 cases (1.5%), and remained upper eyelid notch with mild ptosis in 8 cases (3.0%), and lower eyelid laxity in 2 cases (0.8%). In 5 cases (1.9%), implant exposure developed: 3 cases were resolved with conservative therapy, and 2 cases with surgical revision of the socket. 2 cases experienced persistent pyogenic discharge in conjunctival sac, and the implant was took out because the discharge couldn't control with local and systemic antibiotic therapy. The motility is excellent in 148 cases and good in 11 cases after the primary obital implant, and excellent in 36 cases and good in 68 cases and bad in 3 case after the secondary implant. The difference between the primary and the secondary obital implant is obvious.</p><p><b>CONCLUSIONS</b>It is considered that Medpor orbital implant is a good method of ocular plasty. Although there are some mild postoperative complications, but those can be resolved with conservative therapy or surgical revision. Medpor orbital implant has good histocompatibility, and the operative processes are convenient. Patients are satisfied with appearance and motility. So the Medpor orbital implant is one of the best selection in ocular socket plasty.</p>