HMGB1/SREBP-1 mediated IFN-gamma-induced lipid deposition in mouse mesangial cells / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To explore the possible mechanism of lipid deposition induced by interferon-gamma (IFN-gamma).</p><p><b>METHODS</b>The mouse mesangial cells (MMC) were randomly divided into control group, stimulation group, stimulation + control vector group (sh-HMGB1) and stimulation+ specific sh-vector group (sh-SREBP-1). RT-PCR was used to detect the expression of HMGB1, SREBP-1 and fatty acid synthetase (FAS) mRNA; the protein expression was determined by Western blot.</p><p><b>RESULTS</b>The Oil Red O staining revealed that the mouse mesangial cells showed significant lipid droplet in IFN-gamma group. IFN-gamma up-regulated the expression of HMGB1, SREBP-1, FAS mRNA and protein time-dependently; Transfection of MMC with HMGB1 siRNA resulted in the suppression of SREBP-1, FAS protein levels induced by IFN-gamma, following with decrease of lipid deposition. Stimulation with HMGB1 markedly induced expression of SREBP-1, FAS expression and peaked at 8 h, decreased at 12 h compared with that at 8 h. Sh-SREBP-1 decreased the lipid deposition induced by HMGB1 in MMC.</p><p><b>CONCLUSION</b>IFN-gamma might induce lipid deposition in mouse mesangial cells partly by up-regulating the expression of HMGB1/SREBP-1/FAS.</p>

Pathologic study of circumferential margin involvement in middle and lower rectal cancer / 中华外科杂志

<p><b>OBJECTIVE</b>To study the rule of circumferential margin involvement (CMI) in middle and low rectal cancer and improve its detection rate.</p><p><b>METHODS</b>Pathological large slices stained by HE method was combined with immunohistochemistry to study the CMI of 41 patients with middle and low rectal cancer. There were 20 female and 21 male patients, with an average age of 59.5 years (range, 33 to 77 years).</p><p><b>RESULTS</b>The positive rate of CMI by HE staining was 21.9%. The CMI positive rates of CK20, CDX2 and MMP7 by immunohistochemistry staining was 29.3%, 31.7% and 26.8%, respectively. The positive rate of CMI was 36.6% when combined both HE and immunohistochemistry test, which was significantly higher than those in single methods (all P < 0.05). The positive rate of CMI in poorly differentiated tumor was significantly higher than that in moderately and well-differentiated tumor. The positive rate of CMI in the tumors with a distance of less than 5 cm between the anal verge and the lower tumor margin was significantly higher than that in tumors with the above-mentioned distances of greater than or equal to 5 cm (P < 0.05). According to MMP7 detection, the positive rate of CMI in the group without lymphatic metastasis was significantly lower than that in N1 and N2 group (all P < 0.05). There was no significant correlation between CMI and gender, age, tumor infiltration, lymphatic metastasis, general pathological types and operation methods (P > 0.05).</p><p><b>CONCLUSIONS</b>The positive detection rate of CMI can be improved when combined large slices HE staining and immunohistochemistry. There is significant association between CMI and poorly differentiated tumor, lower location and positive lymphatic metastasis.</p>