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Based on1 H-NMR metabonomics,the effects of Codonopsis pilosula,rice-fried C. pilosula and honey-fried C. pilosula on spleen-asthenia rats were compared,and the mechanism was discussed in this study. The rat model of spleen deficiency was established by weight-bearing swimming and fasting every other day. The effects of different processed products of C. pilosula on the body weight and swimming time of rats were observed. At the end of administration,the gastrocnemius muscle of the right leg of rats was collected and detected by1 H-NMR,and the mechanism of different processed products of C. pilosula in improving spleen deficiency was preliminarily investigated by multivariate statistical analysis. The results showed that C. pilosula,honey-fried C. pilosula and rice-fried C. pilosula could significantly prolong the swimming time( P<0. 05). There was no significant difference in the body weight of rats with spleen deficiency. The results of metabonomics showed that honey-processed C. pilosula could significantly decrease levels of leucine,isoleucine,alanine,acetate,glutamate,succinate,anserine,dimethylamine,dimethylglycine,creatine,phosphorylcholine,glycerophosphorylcholine,taurine,inosine,fumate,hypoxanthine and lactate,but increase levels of glucose,glycine,compared with model group. Therefore,honey-fried C. pilosula has the best efficacy on spleen deficiency syndrome in rats by regulating glycometabolism,amino acid metabolism,lipid metabolism and nucleotide metabolism.
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Animals , Rats , Codonopsis , Magnetic Resonance Spectroscopy , Metabolomics , Proton Magnetic Resonance Spectroscopy , SpleenABSTRACT
@#AIM: To investigate the blood biochemical indexes in total cholesterol, low density lipoprotein cholesterol and blood glucose levels and the correlation of eyegrounds arteriosclerosis.<p>METHODS: This study selected 2017-02/2018-01 97 cases eyegrounds arteriosclerosis patients of our hospital blood biochemical indicator detection as observation group. The other selected line during the same period to our health physical examination results of 98 cases of normal subjects as control group. Detection of two groups of blood biochemical indicators, and then the comparison.<p>RESULTS: The total cholesterol, low density lipoprotein cholesterol, blood glucose and blood pressure levels in observation group were significantly higher than control group(<i>P</i><0.05). When patients blood biochemical routine blood glucose, total cholesterol and low density lipoprotein was high, the degree of eyegrounds arteriosclerosis was higher. By incorporating multiple factors analysis, blood glucose, blood pressure, total cholesterol and low density lipoprotein levels and eyegrounds arteriosclerosis were positively correlated.<p>CONCLUSION: The blood glucose, total cholesterol, blood pressure, and low density lipoprotein level was higher, the eyegrounds arteriosclerosis in patients was more serious, eyegrounds arteriosclerosis level by clinical testing for treatment monitoring, diagnosis, pathological changes.
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Based on 1H NMR metabonomics combined with multivariate statistical analysis, to study the changes of serum endogenous metabolites from immunosuppressive rats induced by cyclophosphamide (CTX), and to screen the best processed products of Codonopsis pilosula, rice-fried Codonopsis pilosula and honey-fried Codonopsis pilosula in improve immunity, and to explore its mechanisms. Methods CTX was administered intraperitoneal (i. p.) to induce the immunosuppressive model. Serum was collected by aorta abdominalis on the last day for detecting immunocyte, immunokine and 1H NMR analysis. Results Codonopsis pilosula and its processed products could increase the numbers of white blood cells and B lymphocytes, as well as the ratio of CD4+ and CD8 + T cells. The regulation effects of honey-fried Codonopsis pilosula and Codonopsis pilosula on IL-2, IgG and sigA were better than those of rice-fried Codonopsis pilosula. Honeyfried Codonopsis pilosula could significantly improve the levels of leucine, isoleucine, alanine, glutamate, glutamine, glucose and valine etc. Conclusions Honeyfried Codonopsis pilosula could play the best role in immunosuppressive rats through regulating valine, leucine and isoleucine metabolism, glutamate and glutamine metabolism, alanine, aspartate and glutamate metabolism.
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Objective:The effect of processing on promoting digestion of Crataegi Fructus was investigated by the method of serum metabonomics based on 1H-NMR and the analysis of traditional biochemical indexes. Method:The dyspepsia model was induced by high protein and calorie diet. The small intestine propulsive rate in mice and the hormone level in rat gastrointestinal tract were used as pharmacodynamic indexes. After administration of Crataegi Fructus, fried and charred Crataegi Fructus, the changes of serum metabolites were analyzed by 1H-NMR technique combined with multivariate statistical analysis, so as to compare the therapeutic effects of each processed products on rats with dyspepsia model. Result:Each processed products group of Crataegi Fructus could improve the ability of intestinal propulsion and gastric emptying, especially in charred Crataegi Fructus group, caused by dyspepsia. The serum metabonomics proved that there were significant metabolic differences between the model group and the blank group. A total of 13 biomarkers related to dyspepsia, covering 3-hydroxybutyric acid, glycerophosphoryl choline (GPC), N-acetylglycoprotein, O-acetylglycoprotein, trimethylamine oxide (TMAO), alanine, acetic acid, glutamic acid, glutamine, creatine, leucine, lactic acid and glucose, were screened on the grounds of VIP value of S-plot, single factor ANOVA and area under receiver operating characteristic curve (ROC curve) (AUC). The metabolite composition of each administration group was the same except that the callback tendency of O-acetylglycoprotein, glutamine and GPC in charred Crataegi Fructus group was closer to that in the blank group. Conclusion:There are differences in the effect of promoting digestion to eliminate stagnation among processed products of Crataegi Fructus, charred Crataegi Fructus is more effective in improving dyspepsia, which may play a role in regulating gastrointestinal motility and energy metabolism.
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Serum pharmacochemistry of traditional Chinese medicine(TCM) is a new subject based on the identification of migrating components in serum after oral administration of TCM.After more than 20 years of development,serum pharmacochemistry of TCM has been widely recognized and applied by researchers.Based on the references related with the serum pharmacochemistry from these years,the research summarized the methods of serum pharmacochemistry of TCM,for example,selection of experimental animals,preparation of gastric irrigation,design of drug administration scheme,method and time of adoption blood,pretreatment of serum containing drug,as well as analysis of serum sample.In addition,the paper will emphatically introduce the application of serum pharmacochemistryof TCM,which includes clarifying the substance basis of Chinese medicine,establishing the quality standard of TCM,and clarifying the compatibility of compound Chinese medicine.At the same time,we will through some existing problems to look forward to the future of serum pharmacochemistryof TCM,so that people can have a comprehensive understanding of serum pharmacochemistryof TCM,hoping to lay a foundation for the further application of this method in TCM research.
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Objective To explore the cellular and humoral immunity in children with recurrent respiratory tract infection(RRI).Methods Fresh peripheral blood samples were treated and run through the flow cytometry,the percentage of lymphocytes subsets were acquired using Simultest software simulaneously. Immunoglobulin IgA,IgG,IgM and complement C3 and C4 were measured.Results The levels of IgG and IgA in the RRI group were lower than the normal reference value in only 1 patient respectively.The level of IgM decreased in 7cases.Complement C3 and C4 decreased in 3 and 1 patients, respectively.The percentage of CD8 in the RRI group was higher than that in healthy control group(P=0),and the CD4/CD8 was obviously lower than that in healthy control group(P=0.002).Conclusions The disorder of cellular and humoral immunity are existing and cell-mediated immune disorder is more obviously in RRI.The immune-modulation agents are necessary in the prevention and treatment for RRI.