ABSTRACT
Minimally invasive treatment is of great significance to gynecological tumors. Laparoendoscopic single-site surgery(LESS)and natural orifice transluminal endoscopic surgery(NOTES)are recent advances in the field of minimally invasive treatment. At present,a variety of gynecological benign surgery can be managed by LESS or NOTES surgery,and achieved good clinical results. Operation for gynecologic malignancies are complicated,and LESS and NOTES surgery are still controversial for the treatment of gynecologic malignancies. At present,domestic and foreign scholars have made some useful attempts to apply NOTES surgery to malignant tumors.Preliminary data have confirmed the feasibility of these procedure,but due to the small operating space,the operation is difficult,and the operation skills still need to be developed.Based on the experience of our hospital's initial exploration,this article briefly explains the current situation,advantages,difficulties and technical experience in the application of NOTES surgery for gynecological malignancies.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate human cytomegalovirus (HCMV) glycoprotein B (gB) genotypes and clinical features in neonates with congenital infections.</p><p><b>METHODS</b>Urine samples were obtained from 67 neonates with HCMV infection confirmed by polymerase chain reaction (PCR). The gB gene fragment was amplified by nested PCR. HCMV gB genotyping was detected by restriction fragment length polymorphism.</p><p><b>RESULTS</b>In all these cases, the most prevalent genotype was gBl (50.7%), followed by gB3 (23.9%), gB2 (17.9%), and gBl/gB3 coinfection (7.5%); gB4 was not found. Moreover, gB1 was more prevalent in infants with liver damage (27/37, 73.0%) than in other symptomatic infants without liver damage (13/30, 43.3%; P < 0.05).</p><p><b>CONCLUSION</b>The gBI genotype is the most prevalent in infants with congenital symptomatic HCMV disease, especially in those with liver damage, followed by genotypes gB3, gB2, and gB4.</p>