ABSTRACT
Objective To investigate the therapeutical effect of Resina draconis. on endometriosis rat model. Methods Totally, 60 adult rats were selected and divided into blank control group( n=10) and blood stasis endometriosis model group( n=50 ) . The blood stasis endometriosis model group was randomly divided into 5 groups after modeling: the Resina draconis. high-, medium-, and low-dose group, Guizhi Fuling group and model control group, 10 rats in each group.The Resina draconis. at 0.50, 0.25 and 0.12 g.kg-1 .d-1 was administered to the rats in the Resina draconis. high-, medium-, and low-dose groups, Guizhi Fuling capsule at 0. 25 g . kg-1 . d-1 to those in Guizhi Fuling group, and the same volume of 0. 5% sodium carboxyl methyl cellulose solution to those in model control group and blank control group. All treatments lasted for 3 weeks.At the end of the treatment, the rats were sacrificed;the volume and weight of ectopic tissue were measured. The blood viscosity was detected with blood rheometer.The concentration of estradiol and progesterone was detected using enzyme-linked immune sorbent assay.The matrix metalloprotein-2 expression in ectopic tissues was determined by RT-PCR. Results High, medium and low dose Resina draconis. significantly reduced the volume and weight of the ectopic tissue, and inhibited MMP-2 expression in ectopic tissues.High-, medium-dose Resina draconis. significantly reduced plasma cutting middle and low shear viscosity of the rats.But the Resina draconis. had no obvious effect on serum estradiol and progesterone concentration. Conclusion Resina draconis. reduces blood viscosity and inhibits MMP-2 expression, which can be used for the treatment of blood stasis type of endometriosis.
ABSTRACT
Objective To study the film￣forming property of film￣forming materials in dragon blood ethanol solution, and provide the reference for screening of film￣forming materials for dragon blood plastics. Methods PVA124,PVA1788, PVP￣K30, EC, MC, PVB were chosen to study the film￣forming property of film￣forming materials based on solubility, formation time, and adhesion. Results PVA1788 and PVP￣K30 mixed in dragon blood ethanol solution in proportion of 4:1 showed good film￣forming property, and the membrane was smooth, uniform and easy to tear off.However, PVA124 and PVP￣K30 mixed in proportion of 4:1 precipitated in dragon blood ethanol solution.EC, MC and PVB were not easy to tear off the membrane. Conclusion PVA1788 and PVP￣K30 mixed in proportion of 4:1 and added with propylene glycol is the optimal choice of film￣forming material for dragon blood spray film.
ABSTRACT
Objective To study the quality control methods for dragon's blood spraying film agent. Methods The pH value and viscosity of dragon's blood spraying film agent were detected. Drug dispersed homogeneous degree and particle sizes were determined with Nano Particle Size Analyzer and microscope. Content of Loureirin B was measured by Ultra Performance Liquid-Chromtography (UPLC). UPLC was performed on Waters C18 column (2. 1 mm×100 mm,1. 7 μm), the wavelength was 280 nm, the column temperature was 40 ℃ , and the mobile phase was 0. 1% formic acid aqueous solution and acetonitrile, and the flow rate was 0. 8 mL·min-1 . Results The pH value and viscosity of dragon's blood spraying film agent were stable, drug dispersion was homogeneous, and particle size of the drug was tiny. The concentration of Loureirin B had a good linear relationship in the range of 15. 51-77. 54 μg. Conclusion This method can be accurately controlled, has good stability and repeatability, and can fully control quality of dragon's blood spraying film agent.