ABSTRACT
Objective:To investigate the methylation status of O6-methylguanine-DNA methyltransferase (MGMT) in meningioma tissue and its effect on the growth and metastasis of human meningioma cell line IOMM-Lee cells.Methods:The specimens of 34 patients with meningioma were collected. Methylation-specific polymerase chain reaction (MSP) method was used to detect MGMT methylation in meningioma tissue and normal brain tissue; immunohistochemical staining was used to detect the expression of MGMT in meningioma tissue and normal brain tissue. The cultured human glioma cell line IOMM-Lee cells were divided into blank control group (normal cultured IOMM-Lee cells), negative control group (empty vector virus transfected IOMM-Lee cells) and RNAi lentivirus transfection group (transfected with RNAi lentivirus vector to down-regulate the expression of MGMT). The expression of MGMT in IOMM-Lee cells was silenced by RNAi technology. The expression levels of MGMT mRNA and protein in cells were detected by real-time fluorescence quantitative PCR and Western blot. The proliferation activity of cells was detected by cell counting kit-8 (CCK-8) test, the colony forming ability was detected by cell clone formation test, and the invasion and migration ability of cells in each group were detected by Transwell test and cell scratch test.Results:The methylation of MGMT in meningioma tissue reached 88.23% (30/34). MGMT methylation was not detected in normal brain tissue; the staining intensity of MGMT in meningioma tissue was significantly higher than that in normal brain tissue. Compared with the blank control group and the negative control group, the relative expression of MGMT mRNA and protein in IOMM-Lee cells of the RNAi lentiviral transfection group were significantly decreased (all P<0.05). After 24, 48 and 72 hours of transfection, the proliferation activity of IOMM-Lee cells decreased significantly (all P<0.05), with reduced number of cell clone formation and cell invasion (all P<0.05). The rate of scar healing decreased significantly ( P<0.05). Conclusions:MGMT is mostly hypermethylated in human meningiomas. Silencing the expression of MGMT in meningiomas can inhibit the growth and metastasis of meningiomas.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the treatment of breast sclerosis caused by capsular contracture after augmentation mammaplasty, and preventive measures after the treatment to avoid the recurrence.</p><p><b>METHODS</b>With the incision through periareola, a part of the fibrous capsule and fibrillate tissue which is between the base of the capsula and the wall of the capsula were resected in the shape of wedge. At the same time, the wall of the capsular cavity was incised in radiant shape to relax it. After operation, the early and effective massage should be fully used.</p><p><b>RESULTS</b>17 case of 26 case were given follow-up over half a year, the results were satisfactory.</p><p><b>CONCLUSIONS</b>After capsular contracture was relaxed and a part of the fibrous capsula between the base and the wall of the capsular cavity was resected in the shape of wedge, the prosthesis can be moved smoothly in the suitable cavity without any obstruction. In addition to the early and effective massage after operation, the methods might reach the ideal results.</p>