ABSTRACT
Objective To explore the application value of enzyme linked immunosorbent assay for detection of hepatitis B PreS1 antigen and antibody in the diagnosis of hepatitis B.MethodsSeveral common patterns in the results of hepatitis Btwo halfcheck were selected from August 2014 to August 2016 for the sample, the use of hepatitis Btwo half7 commonly used detection model for 140 cases of PreS1 antigen, antibody detection.Enzyme linked immunosorbent assay for detection of PreS1 antigen and antibody.ResultsIn patients with HBeAg in PreS1 antigen positive rate was 87.5%, in patients with positive PreS1 antigen positive rate was 8.6%, HBsAg, HBcAb in patients with positive PreS1 antigen positive 13.3%, in a pure HBsAg in patients with positive PreS1 antigen 4.3%.There was no significant difference in the total positive rate of HBV-DNA and the total positive rate of PreS1, and the total coincidence rate was higher.PreS1 antigen positive correlated with HBsAg, HBeAg and HBcAb positive(P<0.05), and has nothing to do with HBsAb, HBeAb positive.ConclusionHepatitis B PreS1 antigen and antibody detection, can effectively complement the diagnosis of hepatitis B, early detection of hepatitis B infection.
ABSTRACT
OBJECTIVE To investigate the pre-operation and/or pre-transfusion testing on patients to avoid risk of(occupational) infection exposure among medical staff and its significance in prevention of nosocomial infection.METHODS Serum hepatitis B(virus surface) antigen(HBsAg),antibody against hepatitis C virus(anti-HCV),(antibody) against human(immunodeficiency) virus(anti-HIV) and toluidine red unheatedserum test(TRUST) were performed on samples from 10 745 cases of pre-operation and/or pre-transfusion from 2003 to 2004.RESULTS The number and(percentage) of positive reaction for HBsAg,anti-HCV,anti-HIV and TRUST were 1163,(10.81%);21,0.20%;1,0.01%(confirmed by HIV Reference Laboratory,Center for Disease Prevention and(Control) of Zhejiang Province) and 74,(0.69%),respectively.TRUST positivity was further confirmed with TPHA.(Overlapping) infections were mostly observed between HBV and HCV,or,to a less scale,between HBV and syphilis.For HBV infection the(department) of digestive medicine was prevalent(?~2=4.77,P