ABSTRACT
<p><b>OBJECTIVE</b>By examining the colony growth rate, colony texture, substrate mycelium color and separation method of 40 anamorph strains of Shiraia bambusicola to investigate the relationship between these indexes and content of hypocrellin A.</p><p><b>METHOD</b>In UV-spectrophotometry and microscopic observation were applied in the experiments. The results were analyzed by the clustering and variance analysis.</p><p><b>RESULT</b>SPSS clustering analysis showed that the colony characters of S. bambusicola anamorph strains had significant differences with hypocrellin A content.</p><p><b>CONCLUSION</b>In the tested conditions, substrate mycelium color of S. bambusicola anamophs has the most distinct correlation with the yield of hypocrellin A.</p>
Subject(s)
Ascomycota , Metabolism , Cluster Analysis , Mycelium , Metabolism , Perylene , Metabolism , Pigmentation , Quinones , Metabolism , Spectrophotometry, UltravioletABSTRACT
<p><b>OBJECTIVE</b>To isolate and identify the anamorph of Shiraia bambusicola.</p><p><b>METHOD</b>Fungus strains were isolated from mature ascospores and stroma. They were identified by means of morphological identification including colony and microscope characteristic, the molecular identification was done by 5.8S-ITS rDNA.</p><p><b>RESULT</b>The strains GZDXIFR-171 and GZDXIFR-181 were isolated and obtained with the separation of different methods, which had the same colony morphology. With the universal primers of the ITS1-5.8S rDNA-ITS2, the 5.8S-ITS rDNA sequence of GZDXIFR-171 and GZDXIFR-181 were obtained by the PCR amplification and sequencing. Compared with the published nucleotide sequence of 5.8S-ITS rDNA in NCBI (National Center for Biotechnology Information), GZDXIFR-171 and GZDXIFR-181 were highly identical with S. bambusicola.</p><p><b>CONCLUSION</b>The isolated strains GZDXIFR-171 and GZDXIFR-181 were confirmed to be the true anamorph of S. bambusicola.</p>
Subject(s)
Ascomycota , Classification , Genetics , DNA, Fungal , Genetics , DNA, Ribosomal Spacer , Genetics , Molecular Sequence Data , Mycological Typing Techniques , Phylogeny , GeneticsABSTRACT
Objective:To evaluate the reliability and accuracy of 3DSS-Ⅱ color structured-light scanning system for scanning and reconstructing the model of abutment tooth.Methods:5 plaster maxillary abutment tooth models were scanned and reconstructed by 3DSS-Ⅱ color structured-light scanning system,and then the 3D shapes of the models were measured by Geomagic 6.0 software and the plaster models were measured by manual with vernier caliper.The data were statistically analyzed.Results:The graphs of point-cloud were obtained from scanning surfaces of abutment tooth models.There were close correlations and no significant differences between different groups(r≈1,P