ABSTRACT
<p><b>AIM</b>To detect the expression of nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) in salivary adenoid cystic carcinoma (SACC) tissues, as well as to determine the correlation between growth factor expression and prognosis in SACC.</p><p><b>METHODOLOGY</b>Medical records of 63 patients surgically treated for SACC between January 1988 and October 2005 were reviewed. Immunohistochemistry was performed to examine the expression of NGF and VEGF in tumor tissues. Kaplan-Meier analysis and Cox's proportional hazard regression model were applied to assess predictors of survival.</p><p><b>RESULTS</b>NGF and VEGF were overexpressed in SACC tissues, compared with those in normal salivary tissues (P < 0.05), and the staining intensity of these two factors was stronger in groups of solid subtype, advanced TNM stage, perineural invasion and recurrence. Patients with high-expression of NGF and VEGF, solid subtype, advanced stage, perineural invasion, recurrence and extended resection alone had worse survival rates (P < 0.05).</p><p><b>CONCLUSION</b>NGF and VEGF are expressed increasingly in the tissues of SACC cases with invasion and metastasis. NGF expression and VEGF expression are independent</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Carcinoma, Adenoid Cystic , Pathology , General Surgery , Cranial Nerves , Pathology , Follow-Up Studies , Immunohistochemistry , Neoadjuvant Therapy , Neoplasm Invasiveness , Neoplasm Recurrence, Local , Pathology , Neoplasm Staging , Nerve Growth Factor , Prognosis , Proportional Hazards Models , Retrospective Studies , Salivary Gland Neoplasms , Pathology , General Surgery , Salivary Glands , Pathology , Salivary Glands, Minor , Pathology , Survival Rate , Vascular Endothelial Growth Factor AABSTRACT
Several novel bioactive components isolated from Chinese medicinal plants will be presented. These include novel maytansinoid tumor, inhibitors, some new ent-kaurane and rosane diterpenoids from Mallotus anomalus Meer et Chun (Euphorbiaceae), as well asnovel insecticide, stemona alkaloids from Stemona parviflora C. H. Wright (Stemonaceae). Both are native plants of Hainan island, Chine. 2D NMR techniques such as mono and hetero-COSY, NOESY, COLOC as well as H-NMR line broadening effect were utilized for structure elucidation. The separation techniques, struture elucidations and bioassay results will be reported.