ABSTRACT
[Abstract] Objective To explore the role of purinergic ligand-gated ion chennel 7 receptor(P2X7R) in cognitive dysfunction in Parkinson’s disease rats. Methods Ninty rats was divided into 5 groups with 6 rats in each group with 3 repeats. The rat model of PD was established by 6-hydroxydopamine (6-OHDA). PD rats were injected with P2X7R agonists 2,3-adenosine 5-triethylammonium triphosphate (BzATP) and antagonists Comassie brilliant blue G (CBBG). The learning and memory ability and pain response of rats in the water maze were measured, and the expression of P2X7 in hippocampus was detected by Real-time PCR and Western blotting. Results Compared with the normal rats, PD rats had slow learning speed and weak memory ability. CBBG improved the learning and memory ability of PD rats, while BzATP decreased the learning and memory ability of PD rats. The result of Real-time PCR showed that compared with the control group, the expression of P2X7R mRNA was the highest in hippocampal tissue, the expression of P2X7R in CBBG group was down-regulated, and that of P2X7R in BzATP group was up-regulated. Compared with the PD group, Western blotting of P2X7R showed that the expression of P2X7 protein increased significantly in BzATP group, while the expression of P2X7 protein was lower in CBBG group. Conclusion Cognitive impairment exists in PD rats. CBBG can improve the learning and memory function of PD rats, and BzATP can inhibit the learning and memory function of PD rats.
ABSTRACT
Data mining method was adopted to collect 159 cases of German migraine patients who live in the TCM-Klinik Bad Kötzting Hospital from January 2013 to March 2015. The general case information was divided into age, gender, profession, characteristics of headache, degree of headache, accompanied symptoms and tongue pulse, and included in corresponding databases. Initial data was standardized and cleaned to get a data form suitable for analysis. SPSS system software was adopted to make a frequency statistics analysis for such variables as gender, age, profession, headache characteristic and accompanied symptoms. The results showed that German migraine cases feature a universality of age, long disease cycle and easy recurrence; And most of the patients were mental workers. There were two common clinical TCM symptoms, namely hyperactivity of liver Yang type and Qi deficiency & phlegm dampness. The common symptoms are forehead tingling, recurrent headache, vomiting and nausea, dark tongue, thin and white fur tongue, wiry pulse and slippery pulse. The disease is related to the pathology of stagnation of Qi, phlegm and blood stasis in liver, spleen, kidney and stomach.