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Cytometry by time-of-flight (CyTOF) is an innovative single-cell analysis technique combining with mass spectrometry principles and flow cytometry. In recent years, CyTOF has been widely used in the study of leukemia treatment for its advantages of multi-parameter, high-throughput, compensation without calculation and diversified data analysis. It can accurately determine intracellular element contents and analyze the changes of unique phenotype and complex signaling pathways of leukemia cells at the single-cell level, to study the mechanism of action of anti-leukemia drugs, discover the potential therapeutic targets of leukemia, assess the therapeutic response and deeply understand the mechanism of leukemia relapse and drug resistance. This article reviews the research progress of CyTOF principle, characteristics, advantages and its application in leukemia treatment.
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10.3969/j.issn.1000-3606.2013.06.005
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Objective To study the effect of ultrashortwave irradiation and chest-wall vibration therapy on serum eosinophil cationic protein(ECP)and percentage of eosinophil(EOS%)in the sputum of children with mild to moderate asthma. Methods A total of 68 children with asthma were divided into a control group and a treatment group.The control group WaS treated with conventional treatment only,while the treatment group was given ultrashortwave irradiation and chest-wall vibration therapy in addition to the conventional treatment.The serum ECP,EOS% in induced sputum,FEV1.0%,and PEF% were measured before and after treatment.The relationships among ECP,EOS%,FEV1.0% and PEF% were analyzed.Results FEV1.0% and PEF% were negatively correlated with serum ECP and EOS% in children with asthma.Compared with the control group,ECP and EOS% were significantly reduced after treatment,while FEV1.0% and PEF% were significantly elevated. Conclusion Uhrashortwave irradiation and chest-wall vibration therapy can improve ventilation by ameliorating airway inflammation and obstruction.
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AIM:To establish the quality standard of the composition of Yuquan Micropill(Radix Rehmanniae,Fructus Schisandrae Chinensis,Radix Puerariae lobatae,etc.). METHODS: The composition of Yuquan Micropill were identified by TLC and the content was determined by HPLC.Puerarin could be separated through Diamonsil~(TM) C_(18) column(5 ?m,250 mm?4.6 mm) with methanol-water(1.2∶3 by volume) as a mobile phase.The flow rate was 1.0 mL/min,the detection wavelength was set at 250 nm and the column temperature was maintained at 40 ℃.?-Schizandrin could be separated through Kromasil KR100-3.5 C_(18) column(100 mm?4.6 mm) with methanol-water(3.5∶1 by volume) as a mobile phase.The flow rate was 0.6 mL/min and the detection wavelength was set at 254 nm. RESULTS: The spots in the TLC were clear and could be well separated,the blank and adjuvant contrast could not be interfered.HPLC showed a good peak form,good resolution and could not be interfered by the foreign matter.The average recovery of puerarin was 99.24%,RSD=0.84%,RSD of precision was 0.95%,RSD of reproducibility was 0.86%,RSD of stability was 0.44%.The average recovery of ?-schizandrin was 99.74%,RSD=0.78%,RSD of precision was 1.89%,RSD of reproducibility was 1.76%,RSD of stability was 1.82%. CONCLUSION: The method is simple and has good resolution,strong specificity and good reproducibility,It can be used for the quality control of the composition of Yuquan Micropill.