The prognostic and influence factors analysis of transanal local resection for stage T1rectal cancer with the distance from anal margin ≤8 cm / 实用肿瘤学杂志

Objective The objective of this was to investipate study the prognostic and influence factors in patients with transanal local resection for stage T1rectal cancer with the distance from anal margin≤8 cm. Methods A fotal of patients with 180 rectal cancer of stage T1with the distance from anal margin≤8 cm from March 2010 to March 2014 were Retrospective analysed,and there were 90 cases received the local resection of rectal cancer as the observation group and 90 patients with the T1stage who under-went radical resection of rectal cancer as the control group. The postoperative recovery effects were compared between the abservation and control groups. The rates of 3-year overall survival and progression-free survival were recorded. The prognostic influence factors of rectal cancer patients at the stage T1with the distance from the anal margin ≤8 cm after transanal local resection were analyzed. Results The operation time,intraoperative blood loss,postoperative anal exhaust time,postoperative hospitalization stay and postoper-ative complications were significantly lower in the observation group than those in the control group(P<0. 05). There were no signifi-cant difference in overall survival and progression free survival between the two groups(χ2= 0. 896,0. 358;P=0. 344,0. 550). Logis-tic multivariate analysis showed that age,degree of differentiation and cutting edge properties were independent risk factors for the prognosis of patients with rectal cancer who were≤8 cm from the anal margin(P<0. 05). Conclusion Transanal local resection for patients with rectal cancer T1stage from distance to anal margin can achieve similar prognostic benefits as radical surgery,and it can promote early recovery after surgery. Age,tumor differentiation and marginal properties are independent factors,which affected the prognosis of the patients undergoing surgery.

Effect of fibroblast growth factor 1 on the proliferating cell nuclear antigen expression in submandibular gland of diabetic mice / 中华口腔医学杂志

Objective@#To examine the proliferating cell nuclear antigen (PCNA) expression in submandibular gland of diabetic mice and to investigate the influence of fibroblast growth factor 1 (FGF-1) on PCNA expression and its possible mechanism.@*Methods@#Sixteen db/db diabetic male mice were randomly divided into diabetic group and diabetic-FGF-1 group (n=8). Eight age-matched db/m mice served as a control group. After FGF-1 was administered intraperitoneally to diabetic-FGF-1 group continuously for 16 weeks, blood glucose and body weight of each mouse in the three groups were detected at 0, 4, 8, 12, 16 weeks. Then the flow rate of saliva in three groups was compared at 0, 8, 16 weeks. At 16 week, bilateral submandibular glands were resected. Then HE staining was performed to observe the histological morphology of submandibular gland and PCNA expression was examined by immunohistochemical staining.@*Results@#Four weeks after administration, the blood glucose in diabetic-FGF-1 group decreased markedly, close to the control group (P>0.05). Weight loss in diabetic-FGF-1 group was noticeable at 8 weeks after administration, but still higher than that in the control group (P<0.05). The flow rate of saliva in diabetic-FGF-1 group increased gradually after administration, which was higher at 8, 16 weeks ([260.1±43.3], [308.5±34.0] mg·min-1·kg-1) respectively than that in the diabetic group at the same time point ([181.8±37.5], [194.9±49.8] mg·min-1·kg-1) (P<0.05). Compared with the control group, submandibular glands in diabetic group significantly atrophied and the glandular atrophy in diabetic-FGF-1 group was alleviated. The submandibular gland index in the control group, diabetic group and diabetic-FGF-1 group were (7.45±0.63), (2.23±0.26), (3.97±0.15) mg/g, respectively (P<0.05). HE staining showed that the histological morphology of submandibular gland in diabetic-FGF-1 group was clearer, and acinar and ductal atrophy were less significant than diabetic group. Immunohistochemistry showed that the rate of PCNA-positive cells in the control group, diabetic group and diabetic-FGF-1 group were (45.23±7.78)%, (11.50±1.69)%, (36.98±6.53)% respectively (P<0.05).@*Conclusions@#FGF-1 can up-regulate the expression of PCNA in submandibular gland of diabetic mice. This effect may be one of the important mechanisms of FGF-1 reversing the structural atrophy and dysfunction of submandibular gland caused by diabetes mellitus.

The prevention of canine osteoradionecrosis of jaws by low-intensity ultrasound / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the preventive effect of low-intensity ultrasound on osteoradionecrosis of jaws (ORNJ).</p><p><b>METHODS</b>Twenty-five canines were randomly divided into experimental group (n=20) and control group (n=5). The canines in experimental group received radiation exposure, and then were randomly subdivided into group A (n=10) and group B (n=10). Control group did not undergo radiotherapy. One month after radiotherapy, the fourth mandibular premolars of all animals were extracted. Group B was immediately treated by low-intensity ultrasound for twenty days, group A and control group did not receive any treatment. Two months after tooth extraction, the formation of ORNJ was determined and the occurrence rate of ORNJ was compared between group A and B. The microstructure of the mandible and changes in microvascular density in group A and B were evaluated and compared with those of control group.</p><p><b>RESULTS</b>All animals in group B and group A developed ORNJ after prophylactic ultrasound was applied for twenty days. Although the imaging examination of bony density of group A and B were lower than normal animals in control group, bone density in group B was significantly better than group A. Micro-CT showed that the trabecular bone volume fraction, trabecular thickness, bone surface/bone volume and trabecular number in group B were respectively (0.187±0.029)%, (0.160±0.039) µm, (12.536±2.558)/mm, (1.227±0.192)/mm, which were all greater than group A [(0.103±0.014)%, (0.069±0.013) µm, (5.598±0.731)/mm, (0.522±0.064)/mm)] (P<0.05).</p><p><b>CONCLUSIONS</b>Although the preventive application of low intensity ultrasound can not prevent the formation of ORNJ, but can significantly improve the symptoms of ORNJ.</p>

The influence of zoledronic acid on vascular endothelial cell / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the influence of zoledronic acid on vascular endothelial cells.</p><p><b>METHODS</b>The influence of zoledronic acid on proliferation, migration and adhesion of vascular endothelial cells were tested with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), cell migration assay and cell adhesion assay. The results of each experimental group were compared with the control group and the data statistically analyzed.</p><p><b>RESULTS</b>In a concentration of 0-0.5 mmol/L, the absorbance value decreased from 0.09 to 0.34 as the drug concentration increased. Scratch test showed that the change of width of scratch before and after 24 hours in control, low, medium and high concentration groups were (38.7 ± 0.42), (35.8 ± 4.17), (19.9 ± 0.57) mm (P < 0.001), (12.5 ± 3.89) mm (P < 0.05). Adhesion test showed that the absorbance value in control, low, medium and high concentration groups were 1.14 ± 0.18, 0.95 ± 0.13, 0.81 ± 0.11 (P < 0.01), 0.67 ± 0.19 (P < 0.001). Comparisons between control and experimental groups were analyzed by t-test and P values < 0.05 were considered statistically significant.</p><p><b>CONCLUSIONS</b>Zoledronic acid inhibits the proliferation, migration and adhesion of vascular endothelial cells.</p>

A case of melanotic neuroectodermal tumor of infancy and review of literature / 实用口腔医学杂志

This paper reports a case of melanotic neuroectodermal tumor of infancy (MNTI)arising in the maxilla of a 3-month-old male infant.The treatment included surgical excision of the lesion with safe margin,curettage of the maxilla and removal of associated developing tooth bud.Microscopically,it proved to be a dual tumor with small,neuroblastic-like cells and larger epithelial cells.Immunohistochemical staining demonstrated epitheloid cells HMB45(+),EMA(+),CK(+);neuroblast-like cells NSE(+),GFAP(+),S-100(+),but both cells Vim(+),CD45(-),Myogenin(-).The 18-mouth follow-up showed no recurrence or metastasis.The related literature was re-viewed.

Pilot-scale production of recombinant plasmid pUDK-HGF / 生物工程学报

pUDK-HGF, the recombinant plasmid DNA encoding human hepatocyte growth factor (HGF), can treat ischaemic disease. A great quantity of pharmaceutical pUDK-HGF is needed. A pilot-scale production process of pUDK-HGF was established based on a new chromatographic media (plasmidselect), including fermentation, cell harvesting, alkaline lysis, ultrafiltration, RNA removing and buffer exchanging on Sephacryl S-1000, capturing supercoiled plasmid DNA with plasmidselect, and removing the salt with Sepharose 6BFF. The process does not use RNase enzyme and toxic solvents.

Serous cystadenoma of the pancreas: a report of 18 cases / 中华普通外科杂志

Objective To investigate the diagnosis and treatment of serous cystadenoma of the pancreas. Methods The clinical data of 18 patients with serous cystadenoma of the pancreas which were admitted into the First Affiliated Hospital of China Medical University from October 1999 to October 2010 were retrospectively analyzed. Results There were 15 females(83.3%) and 3 males (16.7%).Tumors were present in the pancreatic body and tail in 12 cases ( 66. 7% ), in the pancreatic head in 3 cases ( 16. 7% ) and in the pancreatic neck in 3 cases( 16. 7% ). The mean maximum diameter of the tumor was 6. 5 cm. No specific clinical features were indentified. The size of the tumor was significantly correlated with clinical symptoms. CT was main examination with correct diagnosing rate of 61.1%. All 18 patients received surgical resection. Pancreaticoduodenectomy was performed in 3 patients, distal pancreatectomy in 5 cases,spleen-preserving distal pancreatectomy in 5 cases, middle pancreatectomy in 3 cases, and tumor enucleation in 2 cases. Postoperative pancreatic fistula developed in 10 cases (55.6%);Fistula was healed by conservative therapy in all these 10 cases. Postoperative followed up from 6 to 125 months (mean,48. 3months) found no recurrence or metastasis. Conclusions CT was main imaging examination for serous cystadenoma of the pancreas. Surgical resection should be adopted for serous cystadenoma of the pancreas with clinical symptoms but uncertain malignancy.

Prevention of osteoradionecrosis of the mandibule in canine with low intensity ultrasound / 中华创伤杂志

Objective To investigate the repair effect of low intensity ultrasound on radioactive mandibule bone to provide new idea for prevention of osteoradionecrosis of the mandibule after irradiation.Methods The animal models of radioactive mandibular injury were made and treated with low intensity ultrasound.Low intensity ultrasound treatment group ( Group Ⅱ ) and not with low intensity ultrasound treatment group (Group Ⅰ ) were observed by microvessel density detection,micro-CT,ponceau trichrome stain and so on,and compared with control group.Then,the obtained data were statistically analyzed.Results The animal models of radioactive mandibular injury were successfully established.The microvessel density in Group Ⅱ was greater than that in Group Ⅰ.The bone volume fraction,trabecular thickness,bone surface/bone volume and the trabecular number in Group Ⅱ were significantly larger than those in Group Ⅰ (P ＜ 0.05).The ponceau trichrome staining showed that the osteocytes largely disappeared and that the cancellous bone trabecular atrophied in Group Ⅰ,while new bone formation,trabecular and numerous osteoblasts around trabecular were largely observed in Group Ⅱ.Conclusion Low intensity ultrasound has a good recovery effect on bone tissue after irradiation.

Tissue engineering peripheral nerve with TGF-beta repair sciatic nerve defect / 生物医学工程学杂志

The purpose of this investigation was to evaluate the effect of transforming growth factor-beta (TGF-beta) on the efficacy of Schwann cell (SC) and on the repair of peripheral nerve defect. 50 ng x ml(-1) TGF-beta was shown to promote the proliferation of SC by MTT and flow cytometry (FCM) assay, and NGF synthesis in SC culture media was noted to be of significantly higher concentration by ELISA method (P<0.05). SCs mixed with bovine acellular matrix (BAM), fetal bovine serum and media based on definite ratio were injected into polylactideco-glycolide acid (PLGA)guide. 30 SD rats, each had a man-made sciatic nerve defect 15 mm long, were randomly divided into 3 groups: experiment group (PLGA conduit+SC+TGF-beta), control group(PLGA conduit+SC), and autograft group. After 16 weeks, it was demonstrated that the effect of the test group was not significantly different from that of the autograft group, but it was better than that of the control group by means of electrophysiological test and sciatic nerve function index (SFI). TGF-beta can promote not only the proliferation, but also the NGF synthesis of SC obviously. The use of exogenous TGF-beta in the repair of peripheral nerve defect may produce better curative effect.

Establishment of an animal model of blast-fragment combine injury on facial nerve / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study was to establish an animal model to imitate facial nerves injury by explosion.</p><p><b>METHODS</b>The impact was simulated by detonator blast in the distance of 5 cm, 10 cm and 15 cm over the face of dogs under anesthesia and the edge injury of masseters were made by rifle-shot steel ball to imitate segment injury in real explosion. The dogs were killed after different time of injury and the heart, lung, brain and facial nerve were taken to observe the pathological changes in order to evaluating the wound effect in different distances.</p><p><b>RESULTS</b>The animal was injured severely in the distance of 5 cm and could not survive for a long time because of severe damage to brain, heart and lung. The dogs injured at 10 cm could survive after emergency treatment, and there was diffusing hemorrhage in edematous nerve trunk. In the distance of 15 cm, the dogs were injured slightly and, as the distance to the explosive source increased, the local wound became slighter. Under light microscope, the breakage and necrosis of facial nerve fibers could be widely observed, degenerative and necrotic neurons with infiltrating inflammatory cells could be found in the facial nerve nuclei as well.</p><p><b>CONCLUSION</b>The dog's traumatic model established by impact wave and segment in this experiment is an appropriate animal model for the research of explosive effect on facial nerves because of its controllable and repeatable injuring conditions.</p>

THE PATHOLOGY OF DOGS′ FACIAL NERVE AFTER SIMULATED EXPLOSIVE WOUND OF MAXILLOFACIAL REGION / 解放军医学杂志

To observe the injury process and pathological changes of the facial nerve,a primer was detonated at a distance of 10 cm from the face of each of 36 anesthelized dogs to simulate blast injury of the maxillofacial region. At the same time, a tangential wound of masseter was produced by a steel pellet fired with a musket to simulate a shrapnel injury. At different time points after injury, the action potential of the facial nerves was checke d and the pathological changes in axons and neurons of facial nerves were observed after HE and Nissle′s staining,respectively.One day after the injury,the facial nerve axons were found to be disrupted extensively,although the epineurium was still in continuity. There were degeneration and necrosis of neurons with infiltration of inflammatory cells in the facial nerve.One week later, the inflammation began to become milder, and the necrotic neurons were gradually absorbed. Four weeks later, the survived neurons appeared normal, and axons began to regenerate. Meanwhile, electromyography (EMG) showed that the action potential of facial nerve recovered. All the observations suggested that severe indirect injury to the facial nerve trunks in an explosive injury was the main pathological changes which involved an extensive area with severe damage in neurons.

The pathological changes of rat temporomandibular joint under emotional stress / 实用口腔医学杂志

Objective:To observe the influence of emotional stress on the temporomandibular joint(TMJ) of SD rats. Methods:Standard animal model of emotional stress was created by emotion communication emergency box technique in 30 SD rats and foot-shocked in another 30. Control rats were 30 without treatment. The microstructure and ultrastructure of the disc surface, condylar surface and external pterygoid muscle were observed 1, 3 and 5 weeks after emotional stress treatment. Results:Obviously pathological changes were observed in the experimental animals, especially at 3 weeks, involving the fissures on the disc and condylar surface, the collagen fibers were disordered. Electron microscopy observation showed that condylar collagen was exposed. The mitochondria edema and vacuolar degeneration in the external pterygoid muscle were found.At 5 weeks, the condylar cartilage started to recover. Conclusion:Long term emotional stress may lead to pathological changes of the temporomandibular system. The changes can be partly recovered after a certain time of adaption of TMJ.

Treatment of orbital floor fracture with orbital floor mesh plate / 实用口腔医学杂志

Objective:To evaluate the effect of orbital floor mesh plate in the treatment of orbital floor fracture. Methods:27 patients with orbital margin fractures were treated by surgical operation with orbital floor mesh plates or medial wall plates. All patients were observed for 3 months after operation.Results:It was observed that all patients with enophthalmos were cured and the patients with nerve injury recovered satisfactorily.Conclusion:Orbital floor mesh plate is effective in the treatment of orbital floor fracture.

The effect of BMP-2 on the rabbit's facial nerve wound healing:Histological quantitive analysis and ultrastructural observation / 实用口腔医学杂志

Objective: To evaluate the effects of BMP 2 on wound healing of facial nerve. Methods: Facial nerve was crushed in 6 rabbits.1 ng of BMP 2 in 20 ?l of saline was injected around the injured nerve on one side, 20 ?l of saline was injected on the other side as the control.Each two animals were sacrificed 1d,2 and 4 weeks after injury.The nerve samples were studied by histological observation,electron microscopic observation and image pattern analysis.Results:Better healing was observed in BMP 2 treated nerves in 2 and 4 weeks.4 weeks after injury,the axon number on each 100 ?m2 in BMP 2 treated nerves and control ones was 0.946?0.572 and 0.614?0.430(P

Comparative study between the characteristics of facial nerve jnjury resulted from gun-shot and that from blast / 实用口腔医学杂志

Objective: To study the difference between the facial nerve injury by gun-shot and that by blast. Methods: 40 adult dogs were divided into 2 groups, with 20 dogs in each. In the blast group, the impact wave was simulated by detonator blast in the distance of 10 cm over the dog's masseter. At the same time,a steel ball was projected to the dog's face by a rifle to imitate segments in blast. In the gun-shot group, only the steel ball was used. Then the facial nerve was taken at the time of immediateness, 6 hours, 1 day, 3 days and 7 days following the injury for HE or immunocytochemical staining. And the facial nerve conduction velocity was examined 6 hours, 3 days and 7 days after injury.Results: The facial nerve edema was found in both groups just after injury. There was scattering hemorrhage and little hematoma below the spineurium in the gun-shot wound, as well as the diffusing bleeding and larger hematoma in blast wound. In the blast group, the severe edema could be found by light microscope in the whole extracranial facial nerve. Axonal fragments, infiltrating imflammatory cells and few neurofilament protein NF positive axons were observed. In the gun-shot group, only scattering damage was found within 2 cm from the wound track.Conclusion: The facial nerve injury by gun-shot bacomes less severe with the increase of distance from the wound track, but the blast injury is wider and more severe.