ABSTRACT
To observe the effect of uniform and shift rotation culture on the formation and activity of the alginate-chitosan (AC) microencapsulated HepLL immortalized human hepatocytes and HepG2 cells aggregates.AC microcapsulated HepG2 and HepLL cells were randomly divided into two groups. Each group was divided into 3 subgroups according to uniform and shift rotation culture.The size and number of aggregates were observed and measured under laser confocal microscopy and inverted microscope dynamically. The amount of albumin synthesis was detected by ELISA, the clearance of ammonia was detected by colorimetry, and diazepam conversion function was detected by high performance liquid chromatography (HPLC).On day 6, 8, 10, 12, 14 and 16, the number and size of the aggregates, albumin synthesis, diazepam clearance and ammonium clearance increased significantly in shift rotation culture group than in uniform group (all<0.01). The albumin synthesis, diazepam clearance, and ammonium clearance in the microencapsulated HepLL groups were significantly higher than those of HepG2 cells at any time (all<0.01).Shift rotation culture can significantly promote the formation and increase the activity of AC microencapsulated HepLL and HepG2 aggregates, and HepLL cells may be more suitable for bioartificial liver than HepG2.
Subject(s)
Animals , Humans , Albumins , Metabolism , Alginates , Ammonia , Metabolism , Cell Aggregation , Physiology , Cell Culture Techniques , Methods , Cell Line, Transformed , Physiology , Chitosan , Diazepam , Metabolism , Glucuronic Acid , Hep G2 Cells , Cell Biology , Physiology , Hepatocytes , Cell Biology , Physiology , Hexuronic Acids , Liver, Artificial , RotationABSTRACT
Objective To evaluate the effect of levamlodipine intervention in diabetic nephropathy patients which accompanied with hypertension, using the technology of diffusion-weighted imaging (DWI) of functional magnetic resonance (fMRI).Methods A controlled prospective method was taken , and fifty diabetic nephropathy ( phase III) patients which accompanied with hypertension were randomized assigned to two groups of A ( n =26) and B ( n =24).Levamlodipine (2.5 mg qd) was taken by patients of group A and amlodipine (5 mg qd) was taken by patients of group B for 24 weeks, respectively.Two groups both took angiotensinⅡreceptor blockers (ARBs) as the first line antithypertensive agents , their urinary albumin excretion rate (UAER), serum creatinine (sCr), cystatin C (Cys C) , and DWI scanning were detected before and after intervention .The levels of UAER, apparent diffusion coeffi-cient (ADC) value were compared between two groups before and after intervention .During the 24th week, two groups'adverse reac-tion to the medicines and the levels of blood pressure were recorded in each follow-up visit.Results The levels of UAER, systolic blood pressure(SBP), and diastolic blood pressure(DBP) were Significantly lower in group A after 24-week intervention compared to baseline [42.5 (25.3~91.0)μg/min vs 49.2(29.7~96.8)μg/min,(112.6 ±6.4)mmHg vs (135.3 ±7.6)mmHg, (71.4 ± 10.7)mmHg vs (80.3 ±11.6)mmHg, P 0.05).Group A had a better improvement of SBP (ΔSBP) and ADC (ΔADC) after inter-vention compared to group B ( P =0.02,0.01, respectively).The overall adverse reaction incidence was 15.4%(4/26) in group A and 41.7%(10/24)in group B, respectively (χ2 =4.27, P =0.0387).Conclusions For the diabetic nephropathy (phase III) pa-tients accompanied with hypertension , levamlodipine likely showed better effects on reducing comprehensive blood pressure and UAER , improving renal microcirculation , with less overall adverse reaction compared to amlodipine .
ABSTRACT
Objective To investigate the safety and superiority of fast track surgery (FTS)in elderly patients during the perioperative peri-od of laparoscopic cholecystectomy (LC).Methods The clinical data of 124 elderly patients who underwent LC at the Department of Gen-eral Surgery in our institution between January 2010 and March 2014 were assessed,with 62 cases assigned to FTS group and 62 cases to conventional method group.Anal exhaust time,feeding time,off-bed activity time,and length of hospital stay were compared between the two groups,and postoperative results and adverse reactions were recorded.Continuous data were analyzed using the independent-samples t test,and categorical data were compared using the chi-square test.Results Compared with the control method group,patients in the FTS group showed earlier postoperative anal exhaust,feeding,and off-bed activity,a shorter length of hospital stay,and a lower incidence of postoperative cardiovascular and cerebrovascular complications (P<0.05).Conclusion FTS is an effective approach to accelerate rehabil-itation in elderly patients after LC.
ABSTRACT
Objective To investigate the etiology and prevention of venous thrombosis (VT) following laparoscopic cholecystectomy (LC). Methods A retrospective analysis on clinical records of 12 cases of VT following 258 cases of LC in this hospital from January 2002 to June 2004 was carried out. Results [WTBZ]The operation time was 17~58 min (mean, 38 min), and the intraoperative blood loss was 15~100 ml (mean, 45 ml). In 11 cases of superficial vein thrombosis, on a treatment for 6~8 days the symptoms of redness, swelling, heat, and pain vanished. Re-examinations of color Doppler ultrasonography revealed disappearance of thrombosis, and an anticoagulation therapy with Warfarin was given. Follow-ups for 6~12 months found no recurrence. In 1 case of deep femoral vein thrombosis accompanying pulmonary embolism, an anticoagulation therapy was employed for 4 months and a follow-up for 6 months showed no recurrence. Conclusions The development of VT following LC is correlated to patients' high-risk factors, carbon dioxide pneumoperitoneum, and anesthesia administration. Emphasis should be on active countermeasures during peri-operative period.