ABSTRACT
Purpose@#Fibroblast growth factor receptor 4 (FGFR4) plays a critical role in cancer progression involving in tumor proliferation, invasion, and metastasis. This study clarified the role of FGFR4-Arg388 variant in gastric cancer (GC), and more importantly highlighted the possibility of this single nucleotide polymorphism (SNP) as potential therapeutic targets. @*Materials and Methods@#FGFR4 polymorphism was characterized in advanced GC patients to perform statistical analysis. FGFR4-dependent signal pathways involving cell proliferation, invasion, migration, and resistance to oxaliplatin (OXA) in accordance with the SNP were also assessed in transfected GC cell lines. @*Results@#Among 102 GC patients, the FGFR4-Arg388 patients showed significantly higher tumor stage (p=0.047) and worse overall survival (p=0.033) than the Gly388 patients. Immunohistochemical results showed that FGFR4-Arg388 patients were more likely to have higher vimentin (p=0.025) and p-STAT3 (p=0.009) expression compared with FGFR4-Gly388 patients. In transfected GC cells, the overexpression of FGFR4-Arg388 variant increased proliferation and invasion of GC cells, increasing resistance of GC cells to OXA compared with cells overexpressing the Gly388 allele. @*Conclusion@#The exploration mechanism may be through FGFR4-Arg388/STAT3/epithelial to mesenchymal transition axis regulating pivotal oncogenic properties of GC cells. The FGFR4-Arg388 variant may be a biomarker and a candidate target for adjuvant treatment of GC.
ABSTRACT
Objective To encode the protein cystatin M with CST6 gene and construct a CST6-overexpression vector,and transfect it into the gastric cancer cells SGC-7901 in order to observe the effect of cystatin M on the proliferation and migration abilities of SGC-7901 cells.Methods There were three groups in the experiment:pcDNA3.1(+)-CST6 group,pcDNA3.1 (+)group and non-transfected group.RT-PCR and Western blot were used to identify the RNA expression of CST6 in SGC-7901/CST6 cells.The proliferation and migration abilities of the transfected cells were detected by MTT and cell wound healing assay,respectively.Results SGC-7901/CST6 cells stably expressed CST6 gene RNA and cystatin M protein.The proliferation and migration of pcDNA3 .1 (+)-CST6 group cells were reduced compared with those of cells in the other two groups (P<0.05).Conclusion Cystatin M can inhibit the proliferation and migration of SGC-7901 cells.
ABSTRACT
Sentinel lymph node(SLN) is prevalently studied in breast cancer and malignant melanoma as well as being applied in clinical practice.Currently,the study on sentinel lymph node of gastric cancer has gradually been a vital component in the study of diagnosis and treatment of gastric cancer.Sentinel lymph node examination is feasibly applied in clinical T1N0M0 stage gastric cancer or early gastric cancer because of its high accuracy and sensibility.However,the sentinel lymph node examination is not suitable for advanced gastric cancer due to low sensibility and high false negative rate.The article mainly reviewed the advances on SLN tracer,application,micrometastasis,imaging system and so on in gastric cancer.
ABSTRACT
Synovial sarcoma(SS) is a common soft tissue tumor,which origin hasn’t been confirmed. With different morphology and various differentiation, SS is likely to be confused with other soft tissue tumors.Therefore,the rate of misdiagnosis is very high.It is very diffi cult to diagnose SS just relying on symptom,sign,imaging and so on. The article mainly reviews the advances on immunohistochemistry and genetical detection in diagnosis of SS.