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Objective To evaluate the efficacy of Tenghuang-Jiangu tablet combined with osteopeptide injection in the treatment of lumbar hyperosteogeny. Methods A total of 106 patients with lumbar hyperosteogeny were randomly divided into two groups, 53 in each group by digital table method. The control group was treated with osteopeptide injection, and the study group was treated with Tenghuang-Jiangu tablet on the basis of the control group. Both groups were treated for 45 days. The traditional Chinese medcine (TCM) symptoms were scored from pain, numbness, swelling, flexion and extension before and after treatment. The levels of thromboxane B2 (TXB2) and 6-ketone prostaglandin Flα ( 6-Keto-PGFlα ) in serum were detected by ELISA. Adverse reactions during treatment were recorded. Results The total effective rate was 86.8% (46/53) in the study group and 64.2% (34/53) in the control group. There were significant differences between the two groups (χ2=7.338, P=0.007). After treatment, the serum TXB2 (128.01 ± 23.68 pg/ml vs. 172.26 ± 19.33 pg/ml, t=10.539) level in the study group was significantly lower than control group (P<0.01); the serum 6-Keto-PGFlα (36.84 ± 4.96 pg/ml vs. 25.44 ± 4.21 pg/ml, t=12.757) level was significantly higher than control group (P<0.01). After treatment, the scores of pain, numbness, swelling, flexion and extension in the study group were significantly lower than those in the control group (t=10.061, 11.449, 14.921, 15.527, P<0.01). During the treatment period, the incidence of adverse reactions was 17.0% (9/53) in the control group and 22.6% (12/53) in the study group. There was no significant difference between the two groups (χ2=0.534, P=0.465). Conclusions The Tenghuang-Jiangu tablet combined with osteopeptide injection can improve the clinical symptoms of patients with lumbar hyperosteogeny, and regulate the serum levels of TXB2 and 6-Keto-PGFlα.
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Objective To preliminarily evaluate the effect of exosomes from human adipose-derived stem cells (hADSCs) on the migration of epidermal cells.Methods hADSCs were isolated from adipose tissues obtained from a healthy woman after liposuction,and subjected to culture.Exosomes were collected from the culture supernatant of hADSCs by using a modified method combining ultracentrifugatiou with ultrafiltration,and identified and isolated by transmission electron microscopy (TEM),dynamic light scattering (DLS) and Western blot analysis.Some cultured HaCaT cells were divided into several groups to be treated with hydrogen peroxide (H2O2) at different concentrations of 0,50,100,200,250,300,400,500,600,800 μmol/L for 1 hour,and cell counting kit-8 (CCK8) assay was performed to evaluate the effect of H2O2 on the survival rate of HaCaT cells.Some HaCaT cells were classified into 2 groups to be pretreated with phosphate-buffered saline (PBS) (normal group) or 100 μmol/L H2O2 (injury group) for 0.5 hour;then,HaCaT cells in the 2 groups were separately divided into treatment group and control group to be cocultured with exosomes or not.Confocal fluorescence microscopy was conducted to confirm the uptake of PKH26-labelled exosomes by HaCaT cells,scratch assay to estimate the wound healing potential,and Transwell assay to evaluate the migratory activity of HaCaT cells.Statistical analysis was carried out by using one-way analysis of variance for comparison among groups,least significant difference (LSD)-t test for multiple comparisons,and Spearman correlation analysis for analyzing correlations.Results TEM showed that the exosomes isolated from hADSCs were saucer-like nanovesicles with diameters of 60-80 nm.DLS revealed that the purity of the isolated exosomes was 65.88％,and they were stained positively for CD63,Alix and TSG101,which coincided with the basic characteristics of exosomes.CCK8 assay showed that survival rates of HaCaT cells gradually decreased along with the increase of H2O2 concentrations after 1-hour treatment,and were negatively correlated with the concentration of H2O2 (r =-0.91,P ＜ 0.01).Confocal fluorescence microscopy showed that the isolated exosomes could be endocytosed into impaired HaCaT cells.Scratch assay showed that the gap-filling rates at 12 hours were 40.26％ ± 0.64％,69.57％ ± 0.69％,32.28％ ± 0.31％ and 69.62％ ± 1.68％ in the normal control group,normal treatment group,injury control group and injury treatment group respectively,and the injury treatment group showed a significantly increased gap-filling rate compared with the injury control group (t =37.33,P ＜ 0.01).Transwell assay showed that the number of migratory cells per × 10 field was 20.85 ± 4.84,44.8 ± 5.24,14.95 ± 2.58 and 40.05 ± 7.66 in the normal control group,normal treatment group,injury control group and injury treatment group respectively,and was significantly larger in the injury treatment group than in the injury control group (t =25.10,P ＜ 0.01).Conclusion Exosomes isolated from hADSCs can improve the migration of HaCaT cells after oxidative injury.
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Objective@#To preliminarily evaluate the effect of exosomes from human adipose-derived stem cells (hADSCs) on the migration of epidermal cells.@*Methods@#hADSCs were isolated from adipose tissues obtained from a healthy woman after liposuction, and subjected to culture. Exosomes were collected from the culture supernatant of hADSCs by using a modified method combining ultracentrifugation with ultrafiltration, and identified and isolated by transmission electron microscopy (TEM) , dynamic light scattering (DLS) and Western blot analysis. Some cultured HaCaT cells were divided into several groups to be treated with hydrogen peroxide (H2O2) at different concentrations of 0, 50, 100, 200, 250, 300, 400, 500, 600, 800 μmol/L for 1 hour, and cell counting kit-8 (CCK8) assay was performed to evaluate the effect of H2O2 on the survival rate of HaCaT cells. Some HaCaT cells were classified into 2 groups to be pretreated with phosphate-buffered saline (PBS) (normal group) or 100 μmol/L H2O2 (injury group) for 0.5 hour; then, HaCaT cells in the 2 groups were separately divided into treatment group and control group to be co-cultured with exosomes or not. Confocal fluorescence microscopy was conducted to confirm the uptake of PKH26-labelled exosomes by HaCaT cells, scratch assay to estimate the wound healing potential, and Transwell assay to evaluate the migratory activity of HaCaT cells. Statistical analysis was carried out by using one-way analysis of variance for comparison among groups, least significant difference (LSD) -t test for multiple comparisons, and Spearman correlation analysis for analyzing correlations.@*Results@#TEM showed that the exosomes isolated from hADSCs were saucer-like nanovesicles with diameters of 60-80 nm. DLS revealed that the purity of the isolated exosomes was 65.88%, and they were stained positively for CD63, Alix and TSG101, which coincided with the basic characteristics of exosomes. CCK8 assay showed that survival rates of HaCaT cells gradually decreased along with the increase of H2O2 concentrations after 1-hour treatment, and were negatively correlated with the concentration of H2O2 (r= -0.91, P < 0.01) . Confocal fluorescence microscopy showed that the isolated exosomes could be endocytosed into impaired HaCaT cells. Scratch assay showed that the gap-filling rates at 12 hours were 40.26% ± 0.64%, 69.57% ± 0.69%, 32.28% ± 0.31% and 69.62% ± 1.68% in the normal control group, normal treatment group, injury control group and injury treatment group respectively, and the injury treatment group showed a significantly increased gap-filling rate compared with the injury control group (t = 37.33, P < 0.01) . Transwell assay showed that the number of migratory cells per × 10 field was 20.85 ± 4.84, 44.8 ± 5.24, 14.95 ± 2.58 and 40.05 ± 7.66 in the normal control group, normal treatment group, injury control group and injury treatment group respectively, and was significantly larger in the injury treatment group than in the injury control group (t = 25.10, P < 0.01) .@*Conclusion@#Exosomes isolated from hADSCs can improve the migration of HaCaT cells after oxidative injury.
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Objective@#To evaluate the efficacy of Tenghuang-Jiangu tablet combined with osteopeptide injection in the treatment of lumbar hyperosteogeny.@*Methods@#A total of 106 patients with lumbar hyperosteogeny were randomly divided into two groups, 53 in each group by digital table method. The control group was treated with osteopeptide injection, and the study group was treated with Tenghuang-Jiangu tablet on the basis of the control group. Both groups were treated for 45 days. The traditional Chinese medcine (TCM) symptoms were scored from pain, numbness, swelling, flexion and extension before and after treatment. The levels of thromboxane B2 (TXB2) and 6-ketone prostaglandin Flα ( 6-Keto-PGFlα ) in serum were detected by ELISA. Adverse reactions during treatment were recorded.@*Results@#The total effective rate was 86.8% (46/53) in the study group and 64.2% (34/53) in the control group. There were significant differences between the two groups (χ2=7.338, P=0.007). After treatment, the serum TXB2 (128.01 ± 23.68 pg/ml vs. 172.26 ± 19.33 pg/ml, t=10.539) level in the study group was significantly lower than control group (P<0.01); the serum 6-Keto-PGFlα (36.84 ± 4.96 pg/ml vs. 25.44 ± 4.21 pg/ml, t=12.757) level was significantly higher than control group (P<0.01). After treatment, the scores of pain, numbness, swelling, flexion and extension in the study group were significantly lower than those in the control group (t=10.061, 11.449, 14.921, 15.527, P<0.01). During the treatment period, the incidence of adverse reactions was 17.0% (9/53) in the control group and 22.6% (12/53) in the study group. There was no significant difference between the two groups (χ2=0.534, P=0.465).@*Conclusions@#The Tenghuang-Jiangu tablet combined with osteopeptide injection can improve the clinical symptoms of patients with lumbar hyperosteogeny, and regulate the serum levels of TXB2 and 6-Keto-PGFlα.
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Objective To compare the bonding strength of different adhesives to the enamel of mild, moderate and severe dental fluorosis with tensile bonding strength, and to provide the experimental foundation for choosing different adhesives for different degrees of dental fluorosis in clinical practice. Methods At the Affiliated Hospital of Inner Mongolia Medical University, according to the Dean classification standards, one hundred-eighty extracted caries-free and whole human premolars of dental fluorosis were divided into three groups with mild, moderate and severe degree,60 teeth in each group.Each group was divided into two subgroups by random number table method,30 teeth in each subgroup.The emery was used to remove enamel surface 0.5 mm,and then two kinds of bonding adhesives(all-etching adhesive prime&bond NT,self-etching adhesive SE-BOND) were used to bond, pressurized layered composite resin Z350 curing, kept in 37 ℃ distilled water for 24 h. The bonding strength was tested at universal material testing machine, and fracture interface was observated using scanning electron microscope. Results All-etching adhesive had statistical significant influence on the tensile strength of mild, moderate and severe dental fluorosis[(7.82 ± 4.20),(4.79 ± 2.69),(5.04 ± 2.81)MPa,F=4.610,P<0.05], and the tensile strength of mild dental fluorosis was the largest (P < 0.05), the tensile strength between moderate and severe dental fluorosis was not significantly different (P > 0.05). The effect of self-etching adhesive on the tensile strength of mild, moderate and severe dental fluorosis was not statistically significant (F = 0.393, P >0.05). The effect of all-etching adhesive on the tensile strength of mild dental fluorosis was stronger than that of self-etching adhesive(t = 2.197, P < 0.05). Under the scanning electron microscope,the enamel bonding surface of mild dental fluorosis was relatively uniform and compact,with a small amount of crystal disorder,crystal gap slightly widened; the enamel bonding surface of severe dental fluorosis lost seriously enamel, even some of the dentinal tubules exposed, with enamel relatively loose and non uniform, crystal disordered and crystal gap widened significantly, less number of enamel rod. Moderate dental fluorosis was similar to severe dental fluorosis; enamel stripping also occurred under the action of the pulling force but was slighter than severe dental fluorosis enamel. Conclusions ①The effect of all-etching adhesive on the tensile strength of mild dental fluorosis is stronger than that of self-etching adhesive. ②The effect of all-etching adhesive on the tensile strength of mild dental fluorosis is the biggest,there is no statistical significant difference between moderate and severe dental fluorosis.
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Objective To investigate the changes of PTX3,NT -ProBNP level and the correlation between them and the prognosis of the patients with AMI combined T2DM.Methods 143 patients with primary AMI were enrolled,of which 63 patients with type 2 diabetes (observation group)and 80 patients with no diabetes (control group).Plasma PTX3 values were measured with ELISA method and NT -ProBNP was detected by the electrochemi luminescence method.All patients were observed for the occurrence of MACE during hospitalization and 12 months after discharge.The correlation between PTX3,NT -ProBNP concentration and occurrence of MACE in observation group were analyzed.Results The PTX3,NT -ProBNP concentration[(8.95 ±5.06)ng/mL,(1 609 ±1 049)pg/mL]in the observation group were significantly higher than those in the control group[(7.03 ±3.70)ng/mL,(1 198 ± 809)pg/mL](P =0.010,P =0.009);The number(n =26)of patients with occurrence of MACE in the observation group was significantly higher than 15 cases in the control group(P =0.003).In the observation group,the PTX3, NT -ProBNP concentration[(10.98 ±5.45)ng/mL,(2 007 ±1 097)ng/mL]in patients with MACE were signifi-cantly higher than those in patients without MACE[(7.53 ±4.28)ng/mL,(1330 ±930)ng/mL](P =0.007,P =0.010),and in MACE group,the PTX3,NT -ProBNP concentration[(13.88 ±6.84)ng/mL,(2 596 ±1 333)ng/mL] in patients with death weresignificantly higher than those in patients without death[(9.18 ±3.52)ng/mL,(1 639 ± 751)ng/mL](P =0.029,P =0.023).Conclusion More strong chronic inflammatory reaction and serious myocar-dial injury might occur in the patients with AMI combined and T2DM,and those patients would have poorer prognosis. The combined detection of PTX3,NT -ProBNP has an important significance in evaluating of the degree of myocardial damage and the prognosis of the patients with AMI combined T2DM.
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Objective To evaluate the diagnostic value of H-FABP、cTnI and CK-MB for early detection of acute myocardial infarction(AMI).Methods 20 New-Zealand rabbits were randomly divided into 2 groups:surgery group(MI group,n=12) and sham surgery group(control group,n=8).MI group was opened the chests and the anterior descending branches of the left coronary arteries(LAD) were ligated;in sham surgery group the sutures were passed under the LADs without ligation.Blood sampled before surgery and 1,2,3,4,6,8,10 and 24 h after surgery.H-FABP,cTnI and CK-MB were measured by ELISA respectively at the same time.Results The plasma concentrations of H-FABP 1 hour after surgery showed statistical difference between MI group and sham surgery group(P
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Objective To investigate the serum of soluble CD40 ligand(sCD40L) in coronary heart disease and its relationship with serum lipid levels and the extent of coronary stenosis,whether upregulation of CD40L system is related to stability of atherosclerotic plaque in patients with acute coronary syndrome.Methods Enzyme-linked immunosorbent assay(ELISA) was used to measure the level of sCD40L in 64 patients with coronary heart disease(18 with acute myocardial infarction,19 with unstable angina pectoris and the other 27 with stable angina pectoris) and 20 matched healthy controls.Coronary stenosis of 29 patients were assessed by angiographic coronary stenosis morphology.Results sCD40L level was significantly higher in patients with acute coronary syndrome(ACS)((19.8?3.0)、(19.6?4.3)ng/mL in acute myocardial infarction and unstable angina pectoris group,respectively)than that of those with stable coronary heart disease and that of controls((8.3?3.2)ng/mL and(2.6?1.9)ng/mL,respectively P
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Objective To study the effect of 17?-estradial(E-2) on apoptosis of cardiomyocytes induced by norepinephrine(NE) in vitro and its mechanism. Methods The cultured neonatal rat cardiomyocytes were treated with NE(50??mol/L),E-2(10?nmol/L) or NE(50??mol/L)+E-2(10?nmol/L) respectively in serum-free DMEM for 48?h.The morphological changes of myocytes were studied by phase-microscopy and transmission electron microscopy;apoptosis was identified by DNA laddering and apoptotic rate was assayed by flow cytometer;cfos protein in cardiomyocytes was detected using semiquantitative immunofluorescence cytochemistry technique. Results 17?-estradiol inhibited the morphological changes of apoptotic cardiomyocytes induced by norepinephine such as cell atrophy,condensed chromatin clumps against the nuclear envelope associated with swelling of mitochondria and presentation of apoptotic body,DNA laddering disappeared,the apoptotic rate decreased and the expression of c-fos protein inhibited in cardiomyocytes.Conclusion 17?-estradiol can protect cultured cardiomyocytes from apoptosis induced by norepinephrine,its mechanism might associate with suppression of c-fos protein expression.