ABSTRACT
Objective To investigate the prevalence of diphenoxylate abuse and related factors of forced drug abstainer in Gansu province.Methods By using a self-designed questionnaire,an epidemiologic investigation was carried out among 2 108 forced drug abstainer selected from the compulsory isolation detoxification center of Gansu province.A case-control study was conducted to analyze the factors related with diphenoxylate abuse.Results The diphenoxylate abuse rate among forced drug abstainer in Gansu was 19.8％ (406/2 046),ranking first in medical drug abuse.Multiple logistic regression analysis showed that factors as relieving withdrawal symptoms (OR=2.08,95％CI:1.01-4.32),ways to obtain diphenoxylate (other ways:OR=1.00;regular clinic:OR=27.67,95％CI:2.64-289.82;friend:OR=0.01,95％CI:0.01-0.03),degree of euphoria (high:OR=1.00;medium:OR=3.36,95％CI:1.18-9.55;low:OR=26.16,95％CI:10.30-66.42),years of drug abuse (＜5 years:OR=-1.00;10-15 years:OR=2.48,95％CI:1.02-6.04),abuse at home or in friend's house (OR=3.04,95％CI:1.08-8.68),abuse in car (OR=0.05,95％CI:0.00-0.68) and detoxification for the first time (OR=0.61,95％ CI:0.43-0.86) were the possible influencing factors for diphenoxylate abuse.Conclusions The prevalence of diphenoxylate abuse in forced drug abstainer in Gansu was relatively high.Reasons of abusing,the way to obtain diphenoxylate,whether using drug together with friends,degree of euphoria,years of abuse,abuse place and times for detoxification were related factors influencing the abuse of diphenoxylate.
ABSTRACT
Objective To investigate the prevalence of diphenoxylate abuse and related factors of forced drug abstainer in Gansu province.Methods By using a self-designed questionnaire,an epidemiologic investigation was carried out among 2 108 forced drug abstainer selected from the compulsory isolation detoxification center of Gansu province.A case-control study was conducted to analyze the factors related with diphenoxylate abuse.Results The diphenoxylate abuse rate among forced drug abstainer in Gansu was 19.8％ (406/2 046),ranking first in medical drug abuse.Multiple logistic regression analysis showed that factors as relieving withdrawal symptoms (OR=2.08,95％CI:1.01-4.32),ways to obtain diphenoxylate (other ways:OR=1.00;regular clinic:OR=27.67,95％CI:2.64-289.82;friend:OR=0.01,95％CI:0.01-0.03),degree of euphoria (high:OR=1.00;medium:OR=3.36,95％CI:1.18-9.55;low:OR=26.16,95％CI:10.30-66.42),years of drug abuse (＜5 years:OR=-1.00;10-15 years:OR=2.48,95％CI:1.02-6.04),abuse at home or in friend's house (OR=3.04,95％CI:1.08-8.68),abuse in car (OR=0.05,95％CI:0.00-0.68) and detoxification for the first time (OR=0.61,95％ CI:0.43-0.86) were the possible influencing factors for diphenoxylate abuse.Conclusions The prevalence of diphenoxylate abuse in forced drug abstainer in Gansu was relatively high.Reasons of abusing,the way to obtain diphenoxylate,whether using drug together with friends,degree of euphoria,years of abuse,abuse place and times for detoxification were related factors influencing the abuse of diphenoxylate.
ABSTRACT
PAC1 is the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) preferring receptor, which belongs to class B G protein-coupled receptors (GPCR) family. PAC1 mediates the most effects of PACAP as neurotransmitter, neuroregulator and neuroprotectant, while its high expression has close relationship with some physiological and pathological processes such as nerve-injury and tumor. To further understand the function of PAC1, a cell line that expressed inducible PAC1 was constructed to achieve Doxycycline (Dox) dependent expression of PAC1 in CHO (Chinese hamster ovary) cell using the improved Tet (tetracycline)-on Advanced System. First, the PAC1-EYFP fusion gene composed of PAC1 gene and gene encoding EYFP (enhanced yellow fluorescent protein) was sub-cloned to the tetracycline response element pTRE-Tight vector to construct the recombinant vector pEYFP-PAC1-EYFP by double enzyme digestion. Second, the tetracycline regulation components pTet-On advanced vector and the response element pTRE-PAC1-EYFP vector were both introduced into CHO cells successively and the positive clones were screened with G418 and hygromycin respectively. Third, the controlled expression of PAC1-EYFP in CHO was induced by tetracycline analogues Dox in different concentrations and the different levels of receptor PAC1-EYFP were detected. The results of fluorescence analysis and western blotting show that the cell strain with Dox dependent expression of PAC1-EYFP named PAC1-Tet-CHO was obtained. Moreover, in PAC1-Tet-CHO cells the expression of PAC1-EYFP was induced by Dox in a dose-dependent manner. The inducible expression of PAC1 still was stable after sub-culturing for more than 10 passages. It was also found by MTT assay that the higher expression level of PAC1 endowed the cells with higher proliferative viabilities. The construction of controlled expression system of PAC1 will lay a foundation for the further research on PAC1 profiles.