ABSTRACT
Objective: To investigate the chemical constituents of Croton crassifolius. Methods: A number of methods, such as extraction, chromatography on silica gel, and recrystallization, were utilized to isolate and purify the ethanol extract from C. crassifolius, and the structures of the compounds isolated were identified by physicochemical properties and spectral analyses. Results: Ten compounds were isolated and identified as cyperenoic acid (1), β-sitosterol (2), lupeol (3), chettaphanin II (4), 9-[2-(2(5H)-furanone-4-yl) ethyl]-4, 8, 9-trimethyl-1, 2, 3, 4, 5, 6, 7, 8-octahydro-naphthalene-4-carboxylic acid (5), acetyl aleuritolic acid (6), epitaraxerol (7), 9-[2-(2(5H)-furanone-4-yl) ethyl]-4, 8, 9-trimethyl-1, 2, 3, 4, 5, 6, 7, 8-octahydronaphthalene-4-carboxylic ester (8), teucvidin (9), and stigmasterol (10). Conclusion: Compounds 5 and 8 are newly-discovered compounds, named as crassifolius acid and crassifolius ester, and compounds 4, 6, and 7 are isolated from this plant for the first time.
ABSTRACT
<p><b>OBJECTIVE</b>To probe into the clinical effect of needle-pricking therapy for treatment of polycystic ovarial syndrome.</p><p><b>METHODS</b>One hondred and twenty-one cases of polycystic ovarial syndrome were divided into a needle-pricking therapy group of 61 cases and a medication group of 60 cases with randomized and controlled method. The needle-pricking therapy group were treated by needle-pricking therapy at sacral plexus stimulating points on both sides of the spine and lateral points of Dazhui (CV 14), and the medication group by oral administration of domiphen and intramuscular injection of chorionic gonadotropin (HCG). Levels of hormones and symptoms in the patients before treatment, after treatment of 3 cycles and at the sixth cycle after treatment were investigated.</p><p><b>RESULTS</b>After treatment of 3 cycles, the level of hormone and B type ultrasound examination were significantly improved in the two groups (P < 0.01). At the sixth cycle after treatment, the conditions of the patients in the medication group were returned to the original levels before treatment, while the conditions in the needle-pricking therapy group still kept at the post-therapeutic level, and their menstruation and ovulation restored to normal state, and the ovulation mucosa and the pregnancy rate were significantly higher than those in the medication group (all P < 0.01).</p><p><b>CONCLUSION</b>Needle-pricking therapy has obvious effect on polycystic ovarial syndrome, and has a good long-term therapeutic effect.</p>