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This article was aimed to study enhancing efficacy and reducing toxicity on anti-tumor from total flavonoids and total polysaccharides in Taxus mairei compatibility with taxol. MTT assay was performed to analyze the cell proliferation and apoptosis of 4T1, A549, MCF-7 tumor cells after treatment of total flavonoids and total polysaccharides in T. mairei combined with taxol at different concentrations. Isobolograms was used to investigate the behavior of combination therapy. Therapy experiments were conducted on ICR mice bearing S180 sarcoma. The tumor weight was measured to calculate inhibition rate according to uniform design. Blood cells indicators were detected by blood cell analyzer. The results showed that total polysaccharides and total flavonoids can enhance the inhibition effect on 4T1, A549 cells by taxol. Total polysaccharides can enhance the inhibition effect of taxol to MCF-7 in vitro, and S180 sarcoma-bearing mice in vivo. It reduced the toxic effects of anti-tumor effect of taxol in vivo. It was concluded that taxol can achieve the effect of enhancing efficacy and reducing toxicity through combining with total flavonoids and total polysaccharides in T. mairei.
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[Objective]To research the effect of acetic ether extract from T. ramosissima on the skin-wounded mice. [Methods]The acetic ether extract of T. ramosissima was administrated to skin-wounded mice by gavage methods. [Results] The experimental groups in different dosages had significant difference ,compared to the control group, with the areas of wound healing 17.68 mm2, 17.90 mm2, 20.18 mm2 and 31.48 mm2,after the acetic ether extract of T. ramosissima administration to skin-wounded mice by gavage methods. [Conclusions] It was suggested that the acetic ether extract of T. ramosissima had some protective effects on improving the wound healing.
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[Objective] To determine dormancy mechanism of Taxus mairei(Lemee et Levl.) S.Y.Hu ex Liu Seeds,the best method of dormancy breaking.[Method]The seeds were handled by lotic water or excitatory autacoid or cold-warm-cold stratification treatment or cold-warm-cold stratification treatment with excitatory autacoid,then compare withgermination inhibition of Brassica campestris seeds handling with water solution from the seeds after four dormancy breaking methods,to dertemine the best dormancy breaking method.[Conclusion] The best damancy breaking method was cold(4℃)—warm(23℃)—cold(4℃) stratification treatment with 0.05% gibberellic acid for 120 days.
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[Objective] To measure the nitrogen content in Agrocybe cylindracea R.Maire,compare the advantages of Kai's Nitrogen-testing method and Spectrophotometric method.[Method]Take Kai's Nitrogen-testing method and Spectrophotometric method.[Result]The two methods were almost the same in the measurement of nitrogen,i.e.30.70mg/g,which had no marked difference by t test.[Conclusion]Spectrophotometric method is simple and convenient for operation,quickly and correctly,and can be used for measurement of total nitrogen.
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Objective To study the antitussive and antiasthmatic effect of compound liquor from Tremella aurantialba. Methods The mice cough model induced by ammonia, guinea pig cough model induced by acitric acid,guinea pig asthma model induced by histamine and acetylcholine, guinea pig asthma model induced by ovalbumin were used in this experiment. Results The compound liquor from Tremella aurantialba could significantly prolong the tussive delitescence,decreas the tussive times of mice and guinea pigs;could significantly prolong the asthmatic delitescence. Conclusions The antitussive and antiasthmatic effects of compound liquor from Tremella aurantialba was obvious.
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The ideological education of college students based on human being should pay attention to students’ full development,center on their main position in the ideological and political education,respect and embody students' individual difference and settle their practical problems.
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The Retrieval Table of Medicinal Plants is a key tool for identifying plants catogories;under years of teaching experience of medicinal plants and field classification and authentication,it expounds the table,compilation and notes of the said table,and makes examples for application.
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[Objective]Explore the asthma-relieving of Pingchuang Capsule on asthma guinea pigs, its mechanism as well. [Method] Observe the sensitized guinea pigs' changes on latent period of asthma and liver function. [Result] Pingchuang Capsule can markedly prolong the latent period of guinea pigs(P
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Objective To detect the content of polysaccharides in Radix and Rootlet ophiopogonins. Methods Phenylhydrate-sulfuric acid method was used. Results The maximum absorption wavelength was 485 nm. Linear range was 0.101～1.012 mg/L (A=0.826 7C+0.016 3, r=0.999 2). The polysaccharide content in Radix ophiopogonis was 0.301 6 mg/g, and in Rootlet ophiopogonis was 0.359 4 mg/g. The average recovery rate was 100.30% and RSD was 3.14%. Conclusion The polysaccharide content in Rootlet Ophiopogonis was not lower than in Radix Ophiopogonis. It deserves to be more developed and researched.
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To investigate the historical origins of folk herbal medicine "Tian qie er",the origin of "Tian qie er" was confirmed by textural,botanical characteristics research,and field survey.The"Tian qie er" which was originally recorded in"L? Chan Yan Ben Cao"should be "Longzhu"(Tubocapsicum anomalum) of Solanaceae family.