ABSTRACT
The present study was carried out to evaluate the protective role of kombucha mushroom (KM) tea on cytotoxicity induced by phenol (PHE) in mice. We used weight gain and micronucleus (MN) frequency as indicators of cytotoxicity, and supported these parameters with pathological findings. The animals were randomly divided into seven groups: (Group I) only tap water (Group II) 1000 -l kg-1 b. wt KM– tea, (Group III) 35 mg kg-1 body wt. PHE (Group IV) 35 mg kg-1 body wt. PHE + 250 -l kg-1 b. wt KM–tea (Group V) 35 mg kg-1 b. wt PHE + 500 -l kg-1 b. wt KM–tea (Group VI) 35 mg kg-1 b. wt PHE + 750 -l kg-1 b. wt KM–tea, (Group VII) 35 mg kg-1 b. wt PHE + 1000 -l kg-1 b. wt KM–tea, for 20 consecutive days by oral gavage. The results indicated that all KM–tea supplemented mice showed a lower MN frequency than erythrocytes in only PHE–treated group. There was an observable regression on account of lesions in tissues of mice supplemented with different doses of KM–tea in histopathological observations. In conclusion, the KM–tea supplementation decreases cytotoxicity induced by PHE and its protective role is dose-dependent.
ABSTRACT
The Melet is one of Turkey’s economically important rivers. Most of the petroleum plants are located at fairly nearby of the river. This situation is considered as main source of heavy metal pollution in the river. The present study was designed to evaluate the protective role of Ginkgo biloba (GB) on cytotoxicity induced by petroleum wastewater in Vicia faba root tip cells. For this aim, we used the germination percentage, root length, weight gain and micronucleus (MN) frequency as indicators of cytotoxicity. Additionally to the cytological analysis, lipid peroxidation analyses were also performed in V. faba roots. Heavy metal concentrations in wastewater were measured by atomic absorption spectrophotometer (AAS). The V. faba seeds were divided into six groups. They were treated with petroleum wastewater and 10, 20 and 30 /M doses of GB. As a result, the mean concentrations of heavy metals in wastewater were observed in the order: Pb>Al>Ni>Cr>Fe>Cu>Zn>Cd. The highest germination percentage was observed in the seeds of the control and positive control groups (in proportion as 98 and 96%, respectively). Wastewater treatment caused a significant decrease in the germination percentage of Group III (in proportion as 44%). The highest root length and weight gain were observed in the seeds of the control and positive control groups at the end of the experimental period. The least root length and weight gain were observed in the seeds of Group III treated with wastewater alone. In the control group, the final weights of all the seeds increased about 4.08 g according to initial weight. The root lengths of the control seeds were measured as 6.80 cm at the end of the experimental period. The final weights of the seeds exposed to wastewater alone increased about 0.90 g according to initial weight. Besides, there was a significantly increase in the MDA levels of the roots exposed to wastewater. Heavy metals in wastewater significantly affected the MDA production indicating lipid peroxidation. But, GB-treatment caused amelioration in indices of the germination percentage, root length, weight gain, MN frequency and lipid peroxidation when compared with group III. Each dose of GB provided protection against wastewater toxicity, and its strongest protective effect observed at dose of 30 /M. In vivo results showed that GB is a potential protector against toxicity induced by petroleum wastewater, and its protective role is dose-dependent.
ABSTRACT
The present study was carried out to evaluate the protective role of lycopene on cytotoxicity induced by mercury in albino mice. The animals were randomly divided into seven groups. Group I (control) were treated with tap water, Group II (positive control) were treated with 20 mg kg-1 d-1 lycopene, Group III were treated with 10 mg kg-1 body weight mercury, Group IV were treated with 10 mg kg-1 body weight mercury + 5 mg kg-1 d-1 lycopene, Group V were treated with 10 mg kg-1 body weight mercury + 10 mg kg-1 d-1 lycopene, Group VI were treated with 10 mg kg-1 body weight mercury + 15 mg kg-1 d-1 lycopene, Group VII were treated with 10 mg kg-1 body weight mercury + 20 mg kg-1 d-1 lycopene once a day for 20 consecutive days by oral gavage. The initial and final weights of all mice were measured by sensitive balance in order to investigate the effect of mercury and lycopene on the body weight of mice. Then, MN slides were prepared using the standard MN assay technique with Giemsa staining from erythrocyte cells of each mouse and were scored using binocular light microscope (Japan, Olympus BX 51). The results indicated that, all lycopene-supplemented lymphocytes showed a lower MN frequency than lymphocytes in only mercury-treated group. It was seen that lycopene had protective effect on MN particularly at 20 mg kg-1 d-1 dose when compared with the other doses. Besides, weight gain increased depending on dose of applied lycopene when compared with only mercury-treated group. In histopathological examinations, although it has been observed severe changes such as hemorrhage, hepatocyte degeneration and tubular degeneration of kidney in only mercury-treated group, there was an observable regression on the severity and account of these lesions in tissues of mice supplemented with different doses of lycopene. In vivo results showed that the lycopene supplementation decreases cytotoxicity induced by mercury and its protective role is dose-dependent.