ABSTRACT
To investigate the effect of chrysin on apoptosis of oral squamous carcinoma KB cell line and the possible mechanisms, and to provide new ideas for the treatment of oral cancer. Methods: Oral cancer KB cells were treated with different concentrations of chrysin (1, 2, 4, 8, 16, and 32 μmol/L) for 24 h. Cell proliferation was detected by MMT assay; apoptosis was detected by flow cytometry; the activity of caspase-3/7 was detected by chemiluminescent assay; mitochondrial membrane potential in KB cells was determined by JC-1 assay; and Western blotting was used to determine the activation of protein kinase B (AKT) and phosphoinositide-3-kinase (PI3K). Results: Chrysin inhibited the proliferation of KB cells in a concentration-dependent manner, accompanied by increase in apoptosis of KB cells, activation of caspase-3/7, decrease in mitochondrial membrane potential, and suppression of the phosphorylation of AKT and PI3K. Conclusion: The effect of chrysin on KB cell apoptosis may be related to mitochondrial dysfunction and inhibition of PI3K/AKT pathway.
Subject(s)
Humans , Apoptosis , Carcinoma, Squamous Cell , Flavonoids , KB Cells , Mouth Neoplasms , Phosphatidylinositol 3-Kinases , Signal TransductionABSTRACT
Objective:to study the relat onship between personality and mental health in retired military officers Method:178 retired military officers were assessed with EPQ and SCL-90 Result:1 The stable personality type,especially introversive stable type was the most common type in our sample 2 Total score and subscores of SCL-90 were lower than Chinese norm The subscore of depression increased with age,while other subscores had no this trend 3 SCL-90 score was significant different between officers of different types of personality,those with stable personality had lower scores of SCL-90 than those with unstable type Conclusion:mental health of retired military officers is good,most of them belong to introversive stable type in personality