ABSTRACT
Objective To investigate the immunomodulation of PCF on the immunosuppressed mice. Methods The immunosuppressed model was established by injection of dexamethasone (DEX) intraperitoneally. Effects of PCF on the immunity were studied by the observation of morphology of immune organ, the analysis of T lymphocytes subsets and the measurement of lymphoproliferative ability. Results PCF could markedly improve the low immunoreaction of immunosuppressed mice caused by DEX. It was showed that the white pulp of spleen was enlarged, the quantity of T lymphocytes of the peripheral blood was increased and the response of lymphoproliferation to ConA was enhanced in the test group compared to those in the model group. Conclusion PCF had the abilities to up-regulate the immunity in vivo,which may be due to its influences on the peripheral mature immunocytes.
ABSTRACT
Objective To study the protective effects of th e polypeptides isolated from Chlamys farreri(PCF)against ultraviolet B radiatins.Methods An oxidative damage model of HeLa epi thelial cell line irradiated by ultraviolet B(UVB 7.15?10 -5 J /cm 2 )was established.The HeLa cells were randomly divided into six groups:control group,UVB radiated groups(radiated control,0.5%PCF,1%PCF,2%PCF,1%Vit C).The enzymic activities of HeLa cells were tested by MTT methods,the apoptosis rate an d death rate of HeLa epithelial cells and the content of free calcium in those cells were tested by using flow cytometry(FCM).The contents of MDA and the activities of GSH-Px ,SOD,CAT in the supernatants of cells were determin ed by biochemical methods.Results The results indicated that polypept ides fromChlamys farreri could enhance the activities of GSH-Px,SOD,CA T and decrease the contents of MDAin the su pernatants of HeLa cells,increase t he concentration of cellular free calciumin the cells,and decrease the rates of apoptosis and cell death of the cells .Conclusions The PCF has the protective effects on the HeLa cells irradiated by ultraviolet B.The mechanism of th is effects of PCF may be mediated by e-liminating the free radicals,by increasing the activities of antioxidative enzymes to inhibit the oxidativ e damages caused by UVB .