ABSTRACT
Objective: To design an application which can calculate Ki67 and compare its results with the traditional method in gastroenteropancreatic [GEP]-neuroendocrine tumors [NETs]
Study Design: Descriptive analytical study
Place and Duration Of Study: Faculties of Medicine and Technology of Mugla Sitki Kocman University between January 2015 to January 2016
Methodology: A new analyser for detecting the exact percentage of positive cells in images captured from different slides retrospectively selected from hospital records was designed and the concordance with results given by an expert pathologist was compared. Demonstrative slides from randomly selected 50 patients diagnosed as GEP-NETs were stained with Ki67 antibody; and images were captured from the hotspots. The images were then uploaded to the application of the analyser designed for detecting the percentage of Ki67-stained cells
Results: Twenty-seven male [54%] and 23 [46%] female patients with a mean age of 52.3 ±8.80 years were included. According to the pathologist with eye-ball method, 17 cases were grade 1 [34%], 21 cases were grade 2 [42%] and 12 [24%] cases were grade 3. By software, 8 cases were grade 1 [16%], 36 cases were grade 2 [72%] and 6 cases were grade 3 [12%]. Statistical evaluation revealed a kappa value of 0.447 indicating moderate aggreement between the pathologist and the software
Conclusion: The total count of the cells both by the analyser and the pathologist were similar. However, improvements are needed to raise the precision for the detection of positive and negative tumoral cells
ABSTRACT
Objective: To determine the relationship between biochemical recurrence and other histopathological factors in prostate cancer
Study Design: Analytical study
Place and Duration of Study: Pathology and Urology Departments, Izmir Ataturk Training and Research Hospital, between 2001 - 2013
Methodology: 117 cases diagnosed with prostatic adenocarcinoma and treated by radical prostatectomy were reviewed retrospectively for histopathological features; whereas, other prognostic findings were noted. PSA levels and many other histopathological parameters were assessed in order to put forth their effect on biochemical recurrence
Results: PSA level [p<0.001], tumor volume [p<0.001], Gleason score [p<0.001], extraprostatic extension [p<0.001], perineural invasion [p<0.001], ganglion involvement [p=0.040], vascular invasion [p<0.001], positive surgical margins [p<0.001], presence of tertiary pattern [p=0.004] and the involvement of the seminal vesicles [p<0.001] were found to be statistically related to the pathological stage. Age, perineural invasion, high grade tertiary pattern, intraluminal mucin, collagenous micronodules and foamy cytoplasmic changes were unrelated to recurrence
Conclusion: Histopathological features can be helpful in predicting prognosis in prostatic adenocarcinomas. However some of the histopathological factors such as intraluminal mucin and foamy cytoplasmic changes may not reflect high recurrence
Subject(s)
Humans , Male , Middle Aged , Aged , Aged, 80 and over , Recurrence , Adenocarcinoma , Biochemistry , Prostatectomy , Prostate-Specific Antigen , Neoplasm GradingABSTRACT
Nodular fasciitis is a benign, reactive, tumor-like lesion composed of fibroblasts and myofibroblasts. It typically occurs in the extremities and the trunk. Head and neck localization is 13-20%. As it grows rapidly, clinicians frequently misdiagnose it as an aggressive or a malignant lesion. Some lesions show moderate cellularity, mild cellular atypia, and mitosis histologically causing pathologists to over-diagnose the lesion as a malignant tumor. It is important to diagnose nodular fasciitis correctly to avoid unnecessary additional surgery and treatment. We report the case of an 82-year-old woman who was admitted to the emergency department with a one-month history of progressive shortness of breath. We found a mass in the patient's neck, invasive to the trachea, which was the cause of her symptom. Complete radical surgery of the mass with the larynx was impossible due to her general status. The mass was treated by local radiotherapy; however, no regression was seen in the size of the mass. The patient is still on follow-up with only symptomatic medical support for airway obstruction