ABSTRACT
Objective To investigate the relationship between HBV DNA and HBsAg blood group model by analyzing the sero logical and DNA sequences of serum samples.Methods Blood samples from 54 patients were selected from this hospital,collecting blood samples;on the HBV DNA extraction,PCR amplification,purification of PCR products,DNA sequencing,HBV gene analysis and sequence,and the use of bioinformatics software.The results were analyzed and compared the gene mutation.Results The results of PCR amplification,54 specimens in 40 cases showed significant PCR was poor,14 specimens were able to observe the electrophoresis of PCR amplified 1 400 bp specific band;22 cases were HBsAg ELISA positive,14 cases of occult HBV positive,PCR positive amplification for genotype,B genotype accounted for in 81.82 ％ HBsAg ELISA,C gene in occult HBV positive accounted for 78.57％ (P＜0.05);occult HBV gene of 14 strains,6 cases in the S area of the area occurred in the gene sequence of point mutations,including 2 cases in 1 base point mutations,the other 3 cases of base point in 2 sites the emergence of mutation;3 cases of HBV genotype C infected gene point mutation,2 cases of genotype B infected gene point mutation;5 cases were in the 9 site of the "a" family decided to base mutation,A-C mutation.Conclusion Blood screening,detection showed HBsAg negative blood qualified,may still have occult HBV infection and missed the window period,were mainly C type,B type and mutant strain is relatively less,but the mutant is relatively high,may escape the existing screening reagent.
ABSTRACT
Objective To investigate the relationship between HBV DNA and HBsAg blood group model by analyzing the sero logical and DNA sequences of serum samples.Methods Blood samples from 54 patients were selected from this hospital,collecting blood samples;on the HBV DNA extraction,PCR amplification,purification of PCR products,DNA sequencing,HBV gene analysis and sequence,and the use of bioinformatics software.The results were analyzed and compared the gene mutation.Results The results of PCR amplification,54 specimens in 40 cases showed significant PCR was poor,14 specimens were able to observe the electrophoresis of PCR amplified 1 400 bp specific band;22 cases were HBsAg ELISA positive,14 cases of occult HBV positive,PCR positive amplification for genotype,B genotype accounted for in 81.82 ％ HBsAg ELISA,C gene in occult HBV positive accounted for 78.57％ (P＜0.05);occult HBV gene of 14 strains,6 cases in the S area of the area occurred in the gene sequence of point mutations,including 2 cases in 1 base point mutations,the other 3 cases of base point in 2 sites the emergence of mutation;3 cases of HBV genotype C infected gene point mutation,2 cases of genotype B infected gene point mutation;5 cases were in the 9 site of the "a" family decided to base mutation,A-C mutation.Conclusion Blood screening,detection showed HBsAg negative blood qualified,may still have occult HBV infection and missed the window period,were mainly C type,B type and mutant strain is relatively less,but the mutant is relatively high,may escape the existing screening reagent.
ABSTRACT
Objective To improve the quality standard for Chuanxiong by studing the method of discriminating Chuanxiong. Methods Chuanxiong was identified by TLC. The content of ferulic acid in Chuanxiong was determine by HPLC. The Agilent Eclipse XDB-C18 (4.6 mm?150 mm, 5 ?m) was used, mobile phase was methanol-1% acetic acid solution (21∶79), flow rate was 1.0 mL/min, detection wavelength was 320 nm. Results The TLC spots developed were fairly and simply identical. HPLC was accurate and reproducible. Ferulic acid showed a good liner relationship at range of 0.042 5～0.382 5 ?g, the average recovery was 97.10% (RSD=1.31%). Conclusion These methods can be used to control the quality of Chuanxiong effectively.
ABSTRACT
Objective To establish the method for quality standard of Fangzhi Biyan Tablet. Methods Radix Angelicae Dahuricae, Radix Paeoniae Alba, Radix Saposhnikiviae and Radix Glycyrrhizae in the tablet were identified by TLC. The content of Imperatorin in this medicine was determined by HPLC. The Agilent Eclipse XDB-C18 (4.6 mm?250 mm, 5 ?m) was used, mobile phase was methanol-0.1%phosphoric acid solution (65∶35), flow rate was 1.0 mL/min, detection wavelength was 248 nm. Results The TLC spots developed were fairly and simply identical. HPLC was accurate and reproducible. Imperatorin show a good liner relationship at range of 0.076～0.684 ?g, the recovery was 98.63% (RSD=1.52%). Conclusion These methods can be used to control the quality of Fangzhi Biyan Tablet effectively.