ABSTRACT
Objective:To isolate the chemical constituents from lipid-soluble parts of Tibetan Tinospora sinensis and identify the structure of the monomer compounds. Method:Eighty kg of dried rhizomes of T. sinensis were soaked by 80% ethanol (5 times of the dose of crude drug) for 1 hour, boiled under reflux for 1 hour,then and filtered. The above steps were repeated. The two extracts were combined,and the solvent was recovered to obtain a total extract. The obtained extract was extracted with a conventional solvent,and the solution was recovered to obtain petroleum ether extract,methylene chloride extract,ethyl acetate extract,n-butanol extract and water extract. Methylene chloride extract was isolated and purified by silicagel column chromatography,semi-preparative liquid chromatography and SephadexLH-20 chromatography; the chemical structure was identified on the basis of ESI-MS,nuclear magnetic resonance (1H-NMR and 13 C-NMR) spectroscopy data and literatures. Result:Fifteen compounds were isolated and identified respectively as n-dodecanol (1),n-hexacosanol (2),palmitic acid (3),dibutyl phthalate (4),vanillic acid (5),vanillin (6),apocynin (7),tinocordifolioside (8),medioresinol (9),isolariciresinol (10),aurantiamide (11),aurantiamide acetate (12),berberine (13),daucosterol (14),β-sitosterol (15). Conclusion:Except for 3,4,6,14,15,the other 10 compounds were isolated from the plant for the first time.
ABSTRACT
This paper was aimed to investigate the impact of the extraction from raspberry on the Alzheimer disease model protein expression. According to weight, the ovariectomized mice were randomly divided into shame operation group, model group, estrogen positive control group(0.1 g•L⁻¹) and ethyl acetate extraction part control group(in dose of 18 g•kg⁻¹). Each mouse in positive control group was subcutaneous injected of estradiol with 0.2 mL every two days. Raspberry effective parts group were given 0.01 mL•g⁻¹ raspberry ethylacetate extracts, model group and control group were given 0.01 mL•g⁻¹ saline once a day. The drug administration lasted for 32 days. Proteins from mice's hippocampus were extracted, then Nanol-ESI liquid-mass spectrometry system was used for detection and ProteinDiscovery software was used for identification to qualitative analysis different groups of hippocampal proteins by using the software of SIEVE. The results showed that model group compared with the mice of ethyl acetate extraction part control group have 66 differentially expressed proteins including heat shock protein, microtubule protein, protein involved in energy metabolism and protein of brain protection related proteins associated with AD. Those differences protein may be the target that Raspberry prevention and treatment of AD.