ABSTRACT
Aim To investigate whether adiponectin is involved in the protective effect of sevoflurane preconditioning on myocardial ischemia/reperfusion (MI/R) injury. Methods C57 mice were randomly divided into four groups; the Sham Control (sham group), the MI/R-Control (model group), the MI/R-Sevopre (pretreatment group) and the APN-KO-MI/R-Sevopre (knockout pretreatment group). The plasma adiponectin level was detected 24 h after MI/R modeling. Meanwhile, the left ventricular end-systolic diameter, end-diastolic diameter and ejection fraction were measured at 24 h. In addition, TUNEL staining was used to detect the left ventricular end-systolic diameter, end-diastolic diameter and ejection fraction and the morphology and apoptosis of myocardial cells in each group were observed. What' s more, the infarct area was observed by Evans blue TTC staining. Results Compared with sham group, model group had impaired heart function, decreased ejection fraction and increased end-diastolic and end-systolic diameters. Meanwhile, the myocardial infarction area and apoptotic cells significantly increased in model group, and the plasma adiponectin level decreased. However, compared with model group, mice in sevoflurane pretreatment group had improved heart function, decreased myocardial infarction area, decreased apoptotic cells and increased plasma adiponectin levels. And then, compared with pretreatment group, sevoflurane pretreatment in knockout pretreatment group reduced Ml/R injury. Conclusion The sevoflurane pretreatment protects MI/R injury by increasing plasma adiponectin levels.