ABSTRACT
Objective To explore the application of de-correlation algorithm to laser speckle blood flow imaging technique and study the blood flow velocity.Methods The principle and algorithm were analyzed for de-correlation laser speckle blood flow imaging.A laser speckle imaging platform was established,and de-correlation laser speckle blood flow imaging algorithm was used to execute microfluidic target experiment and animal experiment.De-correlation algorithm was applied to obtaining blood flow image and velocity curve of microfluidic target.Cross-correlation curve on the original speckle images were got with cross-correlation algorithm,and the original images corresponding to the peak points were selected to undergo computation with de-correlation algorithm,so that the minute information could be augmented on the blood vessel.Results The de-correlation laser speckle blood flow imaging algorithm could be used to obtain the blood flow information and velocity information during microfluidic target experiment and the animal experiment.Conclusion The de-correlation laser speckle blood flow imaging algorithm is feasible in laser speckle imaging,and has good application prospects.