ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities.</p><p><b>METHODS</b>The ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two-dimensional gel electrophoresis (2-DE)-MALDI-TOF-MS proteomic approach.</p><p><b>RESULTS</b>Thirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix (ECM), cytoskeleton, growth factor, glycolysis, protein metabolism and immune system.</p><p><b>CONCLUSION</b>The data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer.</p>
Subject(s)
Female , Humans , Cell Line, Tumor , Electrophoresis, Gel, Two-Dimensional , Gene Expression Regulation, Neoplastic , Neoplasm Metastasis , Ovarian Neoplasms , Metabolism , Pathology , Proteomics , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
<p><b>OBJECTIVE</b>To conduct a proteomic analysis of human colorectal carcinoma cell lines LS174T and SW480 by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).</p><p><b>METHODS</b>The total proteins of human colorectal carcinoma cell lines LS174T and SW480 were separated with 2-DE using immobilized pH gradient strips and analyzed by MALDI-TOF-MS to obtain peptide mass fingerprints (PMFs). Proteins were identified by using Mascot software to search protein databases.</p><p><b>RESULTS</b>Good resolution 2-DE maps were obtained. Some proteins including immunoglobulin heavy chain variable region (IgVH) and co-stimulatory molecule B7-1 were identified. IgVH and B7-1 were confirmed by electrospray ionization tandem spectrometry (ESI-MS/MS) and immunocytochemistry.</p><p><b>CONCLUSION</b>There are IgVH and B7-1 expressions in human colorectal carcinoma cell lines LS174T and SW480. Results obtained will help to elucidate the mechanisms of tumor immune escape.</p>
Subject(s)
Humans , B7-1 Antigen , Genetics , Cell Line, Transformed , Colorectal Neoplasms , Genetics , Allergy and Immunology , Metabolism , Immunoglobulin Heavy Chains , Genetics , Immunoglobulin Variable Region , Genetics , Neoplasm Proteins , Genetics , Allergy and Immunology , Peptide Mapping , ProteomeABSTRACT
<p><b>OBJECTIVE</b>To investigate the distribution of regulatory T-lymphocytes in the splenocytes cocultured with syngeneic low-immunogenic tumor cells, as compared with that of highly-immunogenic tumor cells, to investigate the mechanism underlining tumor evasion.</p><p><b>METHODS</b>Three different immunogenic tumor cells were cocultured with syngeneic splenocytes individually to mimic cancer immunity in vitro. The proliferation response of splenocytes was measured by thymidine incorporation. The distribution of TR cells, CD4(+) IFN-gamma (+) T cells and CD4(+) IL-10(+) T cells were analyzed by flow cytometry. The secretion of IFN-gamma and IL-10 in supernatants was measured by ELISA assay.</p><p><b>RESULTS</b>The stimulation Index of splenocytes cocultured with syngeneic highly-immunogenic H22 or FBL3 was much higher than that of poorly immunogenic melanoma D5. In each group, stimulation Index of splenocytes cocultured with allogeneic tumor cells was higher than that of the corresponding tumor immunity model. In addition, compared with those of highly-immunogenic tumors, there were more TR, CD4(+)IL-10(+) and less CD4 (+)IFN-gamma(+) T cells in the splenocytes, and higher IL-10 and lower IFN-gamma levels in the supernatant of the splenocytes stimulated with low-immunogenic D5 cells.</p><p><b>CONCLUSION</b>Poorly-immunogenic tumor cells can induce the proliferation of TR cells, which may play an important role in tumor evasion.</p>