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Journal of Forensic Medicine ; (6): 113-114, 2005.
Article in Chinese | WPRIM | ID: wpr-983085

ABSTRACT

OBJECTIVE@#To investigate the relationship between DNA degradeation and postmortem interval.@*METHODS@#dUTP was transferred to 3'terminal of DNA by using terminal deoxynucleotidyl transferase (TDT), then the reminders of dUTP after experimental reaction, as indicator of quantity of DNA degradation, were detected.@*RESULTS@#The reminders of dUTP were decreasing along with the postmortem interval.@*CONCLUSION@#Postmortem DNA degradation may be used in postmortem interval judgment.


Subject(s)
Animals , Male , Rats , Cell Nucleus/metabolism , Clinical Enzyme Tests/methods , DNA/metabolism , DNA Nucleotidylexotransferase/metabolism , Kidney/metabolism , Liver/metabolism , Postmortem Changes , Pyrophosphatases/metabolism , Rats, Sprague-Dawley , Spectrophotometry, Ultraviolet , Spleen/metabolism , Time Factors
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