ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Huanglian Jiedutang (HLJDT) on hippocampal protein expressions in senescence accelerated mouse-prone/8 (SAMP8).</p><p><b>METHOD</b>The 12-month-old senescence accelerated mice (SAM) were divided into three groups: SAM-resistance/1 (SAMR1), SAM-prone/8 (SAMP8) and SAMP8 treated with HLJDT. The effect of HLJDT on expressions of hippocampal proteins was analyzed by two dimensional electrophoresis (2DE) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).</p><p><b>RESULT</b>Compared with same age SAMR1, there were 29 differential expressed hippocampal proteins in SAMP8. After treated with HLJDT, the expressions of 38 hippocampal proteins of SAMP8 were changed significantly. 12 reactive proteins of HLJDT were chosen to be identified by MALDI-TOF-MS and the results were searched in MASCOT database. Among 12 reactive proteins, the expressions of 4 hippocampal proteins which expressed differentially between SAMR1 and SAMP8 could be improved by HLJDT.</p><p><b>CONCLUSION</b>HLJDT may improve the aging of SAMP8 by regulating the expressions of proteins related with energy metabolism, signal transduction, cytoskeletal, amino acid metabolism and so on.</p>
Subject(s)
Animals , Male , Mice , Coptis , Chemistry , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Electrophoresis, Gel, Two-Dimensional , Fructose-Bisphosphate Aldolase , Metabolism , Glutamate-Ammonia Ligase , Metabolism , Hippocampus , Metabolism , Plants, Medicinal , Chemistry , Proteome , Metabolism , Proteomics , Methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
OBJECTIVE: To evaluate the risk factors of colorectal cancer in China. METHODS: The results of 14 case-control studies from 1988 to 2000 were analyzed by means of Meta-analysis. The total numbers of cases and controls were 5034 and 5205 respectively. Dersimonian and Laird random effective models were applied in processing data. RESULTS: Light physical activities and dietary fibers were protective factors (pooled OR<0.8); while histories of fecal mucohemorrhage, chronic diarrhea and bowel polyps were highly associated with colorecatal cancer (pooled OR >4) The stratified results indicated that there were probably some differences between OR values of some factors if using different sources of cases and controls or using different data from the north and south of China. CONCLUSION: Risks of colorectal cancer are significantly associated with the histories of intestinal diseases or relative symptoms, fatty food, psychic attack and family history of cancers. The light physical activities and dietary fibers are probably protective factors.