ABSTRACT
Abstract Purpose: To examine the effect of taxifolin on I/R induced gastric injury in rats using biochemical and histopatholohical methods. Methods: Eighteen albino Wistar male rats equally grouped as; gastric I/R (I/R), 50 mg/kg taxifolin + gastric I/R (TAX+ I/R) and sham operation applied (SHAM). Ischemia induced for 1 hour, and reperfusion induced for 3 hours. Results: Oxidant parameters like, Malondialdehyde (MDA) and Hydroxyguanine (8-OHdG) were higher, whereas total glutathione (tGSH) was lower in the I/R group according to SHAM group, histopathological findings such as marked destruction, edema, and proliferated dilated congested blood vessels were observed severely in the I/R group, whereas there was not any pathological finding except mild dilated congested blood vessels in the TAX+ I/R group. Conclusion: The taxifolin can be clinically beneficial in the treatment of gastric injury due to I/R procedure.
Subject(s)
Animals , Male , Rats , Quercetin/analogs & derivatives , Reperfusion Injury/prevention & control , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Gastric Mucosa/injuries , Oxidation-Reduction/drug effects , Quercetin/therapeutic use , Celiac Artery/surgery , Rats, Wistar , Oxidative Stress/drug effects , Disease Models, Animal , LigationABSTRACT
OBJECTIVES: To determine the serum and tissue levels of markers of impaired oxidative metabolism and correlate these levels with the histopathology and Alvarado score of acute appendicitis patients. METHOD: Sixty-five acute appendicitis patients (mean age, 31.4±12.06 years; male/female, 30/35) and 30 healthy control subjects were studied. The Alvarado score was recorded. Serum samples were obtained before surgery and 12 hours postoperatively to examine the total antioxidant status, total oxidant status, paraoxonase, stimulated paraoxonase, arylesterase, catalase, myeloperoxidase, ceruloplasmin, oxidative stress markers (advanced oxidized protein products and total thiol level) and ischemia-modified albumin. Surgical specimens were also evaluated. RESULTS: The diagnoses were acute appendicitis (n = 37), perforated appendicitis (n = 8), phlegmonous appendicitis (n = 12), perforated+phlegmonous appendicitis (n = 4), or no appendicitis (n = 4). The Alvarado score of the acute appendicitis group was significantly lower than that of the perforated+phlegmonous appendicitis group (p = 0.004). The serum total antioxidant status, total thiol level, advanced oxidized protein products, total oxidant status, catalase, arylesterase, and ischemia-modified albumin levels were significantly different between the acute appendicitis and control groups. There was no correlation between the pathological extent of acute appendicitis and the tissue levels of the markers; additionally, there was no correlation between the tissue and serum levels of any of the parameters. CONCLUSIONS: The imbalance of oxidant/antioxidant systems plays a role in the pathogenesis acute appendicitis. The Alvarado score can successfully predict the presence and extent of acute appendicitis. .
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Appendicitis/metabolism , Oxidative Stress/physiology , Reactive Oxygen Species/analysis , Acute Disease , Appendectomy , Antioxidants/analysis , Aryldialkylphosphatase/analysis , Biomarkers/analysis , Case-Control Studies , Carboxylic Ester Hydrolases/analysis , Prospective Studies , Peroxidases/analysis , Reference Values , Reactive Oxygen Species/metabolism , Statistics, Nonparametric , Serum Albumin/analysisABSTRACT
A introdução de materiais totalmente cerâmicos à base de sílica e das técnicas de cimentação adesiva possibilitou preparos inovadores e estética excepcional no campo da odontologia restauradora. Apesar dos constantes aperfeiçoamentos das propriedades ópticas e mecânicas, as restaurações de cerâmica vítreas ainda estão bastante limitadas a restaurações unitárias, bem como a próteses fixas de espaço protético pequeno, com determinadas restrições, na zona estética. Assim como as propriedades mecânicas das novas cerâmicas de óxido de alta resistência, tais como as cerâmicas de dióxido de zircônia estabilizadas parcialmente, a confiabilidade delas a longo prazo também melhorou significativa-mente, facilitando a aplicação clínica de retentores radiculares de cerâmica pura, de pilares de implante, bem como de infra-estruturas para próteses fixas, aplicáveis também na região posterior. Tendo em vista a variedade sempre crescente de sistemas totalmente cerâmicos, este artigo fornece conceitos de tratamento baseados em estudos clínicos, incluindo a seleção correta do material, dependendo da situação clínica.