Isolation and PC12 cell proliferative protein fraction from pilose antler and its activity / 中草药

Objective The lyophilized pilose antler water extract(PAE) was isolated,and their cell proliferation on PC12 cells was observed.Methods S-200 Size-exclusive gel and DEAE negative ion-exchange liquid chromatograph were employed to fractionate the PAE.SDS-PAGE was employed to analyze the proteins composition of PAE.The protein concentration was determined by Folin-Phenol assay.The proliferation rates of PC12 cells were measured by MTT assay.Results The proliferation rate of PAE on PC12 cells at 13.3 mg/mL was 47%(P

Isolation,purification and structure determination of antifungal antibiotic FW03-1149 produced by marine Micromonospora sp.FIM03-1149 / 中国海洋药物

Objective To study the antifungal metabolites from marine microorganism FIM03-1149.Methods The producing strain was identified by taxonomical studies.The antifungal compound FW03-1149 was extracted by organic solvents and purified by silica gel column and preparative HPLC from the culture broth of FIM 03-1149.The structure of FW03-1149 was determined by MS data analysis and physico-chemical properties.Results and Conclusion The producing strain was named as Micromonospora sp.FIM03-1149.Compound FW03-1149 was determined to be neorustmicin,showed strong antifungal activities.