ABSTRACT
The nervous system is one of the most complicated organ systems in invertebrates and vertebrates. Down syndrome cell adhesion molecule (DSCAM) of the immunoglobulin (Ig) superfamily is expressed widely in the nervous system during embryonic development. Previous studies in Drosophila suggest that Dscam plays important roles in neural development including axon branching, dendritic tiling and cell spacing. However, the function of the mammalian DSCAM gene in the formation of the nervous system remains unclear. Here, we show that Dscam ( del17 ) mutant mice exhibit severe hydrocephalus, decreased motor function and impaired motor learning ability. Our data indicate that the mammalian DSCAM gene is critical for the formation of the central nervous system.
Subject(s)
Animals , Mice , Cell Adhesion Molecules , Genetics , Metabolism , Corpus Callosum , Metabolism , Pathology , Genotype , Hydrocephalus , Genetics , Metabolism , Pathology , Mice, Knockout , Motor Activity , Genetics , Physiology , MutationABSTRACT
Objective To establish a HPLC method for determining Baicalin in Chaigejieji decoction. Method Baicalin was separated on ODS Kromasil C18 column with a mobile phase of methanol-water- phosphoric acid (47∶53∶0.2) and detected at 280 nm with a flow rate of 1.0 mL/min. Results The calibration curve was linear at the range of 0.3373～1.6865 ?g (r =0.9997). The average recovery of Baicalin was 97.87% and RSD was 1.66%. Conclusion It is a simple and accurate method for the determination of Baicalin in Chaigejieji decoction.