ABSTRACT
Objective To assess the therapeutic effect of microwave radiation combined with Adriamycin liposome on liver VX2 tumor in rabbits. Methods VX2 tumor pieces were successfully implanted into liver lobes of rabbits with liver tumors formation. All rabbits were divided randomly into 4 groups (each n=8). Group A underwent transcatheter arterial chemoembolization (TACE) with Adriamycin, group B were treated with Adriamycin liposome, group C were treated with microwave radiation combinate with Adriamycin, and group D were treated with microwave radiation combinate with Adriamycin liposome. Spiral CT scans was performed to measure size of liver tumors at 1 day before operation and 14 days after operation, and liver function was tested at 1 day before operation, 1 day and 7 days after operation. Breathing frequency, heart rate and body temperture were measured before microwave radiation processing of microwave radiation, at 30 min after microwave radiation and 1 h after microwave radiation. All experimental animals were scarficed on the 15th day after operation and followed by pathologic and histologic examination of the tumor and after the operation with correlative comparisons. Results There was no significant difference in volumes and liver function among 4 groups at 1 day before operation. At 14 days after operation, the average tumor volume was (6.02±1.21) cm3 in group A, (5.74±1.43) cm3 in group B, (3.26±0.37) cm3 in group C and (1.89±0.14) cm3 in group D. There was no significant difference of liver function among 4 groups. After microwave radiation the rabbit's vital signs were stable. The intratumoral necrosis was more significant in group D than in the others (P<0.05). Conclusion Adriamycin liposome treatment for liver tumor via vascular interventional method combined with microwave radiation can significantly inhibit the growth of liver VX2 tumor in rabbits. Adriamycin liposome is a kind of effective pracputium in interventional chemoembelization, and the united therapy is safe and acceptable.
ABSTRACT
Objective To investigate the role of dendritic cell (DC) subsets and T cell subpopulations expressing different intracellular cytokines in the pathogenesis of syphilis. Methods Flow cytometry was utilized to measure the frequencies of peripheral DC subsets and IFN-γ-, IL-2-, IL-4-, IL-10-expressing peripheral CD4+ and CD8+ T cell subpopulations in 20 patients with syphilis before and after therapy, and in 15 healthy human controls. Results The frequency of CD11c+ DC was significantly lower in pretreated patients than that in treated patients (P<0.01), but higher than that in the normal controls (P<0.05), and the same was true for the frequency of IFN-γ-expressing T cells, IL-2-expressing T cells, and IL-12-expressing T cells. By contrast, a decrease was noticed in the frequency of IL-4-expressing T cells and IL-10-expressing T cells in pretreated patients compared with that in the normal controls. Furthermore, in patients with syphilis, the frequency of CD11c+ and CD123+ DC was unrelated to the percentage of IFN-γ-expressing and IL-12- expressing T cells (both P>0.05). Conclusions IFN-γ, IL-2 and IL-12 may exert an immunoregnlatory effect in the eliminating of Treponema pallidum, whereas IL-4 and IL-10 may perform an imrnunoinhibitory effect.