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@#Objective To explore 64 row volumetric CT 3D reconstruction in the clinical application of severe craniocerebral injury.Methods 60 patients with craniocerebral injury in our hospital were retrospectively analyzed who accept craniocerebral 64 row volumetric CT 6 to 24 hours after injury. Results All cases got timely diagnosis and treatment, especially for the special parts of brain lesions, ventricle base of skull fractures and pool, and various angles show. Conclusion 64 row volumetric CT reconstruction for the diagnosis of craniocerebral injury shows important significance and may estimate the prognosis in diagnosis, guiding treatment and surgery, reducing misdiagnosis.
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@#Objective To assess the application of 64-row volumetric CT three dimensional image formation to shape the titanium mesh for the skull neoplasty. Methods 40 cases were divided into the shaping before operation group (21 cases), in which the titanium meshes were shaped with the data from 64-row volumetric CT skull three dimensional image formation before operation; and the shaping during operation group (19 cases), in which the titanium meshes were shaped approximately before and exactly during operation. The time of shaping, anaesthesia and the whole operating were compared, and the qualities of the skull plasty were assessed. Results All the skull repaired satisfactorily. The time of shaping, anaesthesia and the whole operating was shorter in shaping before operation group than in shaping during operation group (P<0.05). No complication was observed. Conclusion Application of 64-row volumetric CT three dimensional image formation in the skull neoplasty may reduce the surgery time and risk.
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Objective To investigate the expression and distribution of sialyl Lewis X in colorectal carcinoma and its relationship with carcinogenesis, progression and metastatic proclivity.Methods Microwave LSAB immunohistochemical technique was used to detect the expression of sialyl Lewis X in colorectal carcinoma and in normal mucosa. Immunoelectromicroscopic localization of sialyl Lewis X labelled by colloidal gold was also observed.Results The positive rate of sialyl Lewis X expression in primary colorectal cancer was 92 2%(83/90), and 16 7%(5/30) in normal mucosa. The positive rate was 100% in patients with lymphatic nodes metastasis, compared with that of negative nodes of 82 1% ( P
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Objective To investigate the effect of antigen retrieval by microwave and the expression of CD 44 s and CD 44v6 in human intracranial metastastic tumor and human glioblastomas, and the relationship between the expression of CD 44 adhesion molecules and the invasion or metastasis of these tumors.Methods After antigen retrieval by microwave-0.1%Triton X-100(MW-T) and buffer solution of microwave-citrate salts(MW-CA), CD 44 s and CD 44v6 were used to detect the expression of CD 44 adhesion molecules in 20 normal human brain tissues, 35 human glioblastomas, and 30 intracranial metastastic tumor by microwave-LSAB immunohistochemical staining technique.Results Integral optical density(IOD) value of CD 44 s and CD 44v6 positive cells restored by MW-T and MW-CA was higher significantly than that of without antigen retrieval (P
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ObjectiveTo improve the diagnosis of occult or recurrent inguinal indirect hernias. Methods 47 patients with obscure groin or incisional pain with normal physical examinations were undergone herniography. Results26 patients had hernias confirmed by herniography, including 22 indirect and 4 recurrent hernias. The symptoms disappeared after herniorrhaphy in the 26 patients. Eighteen of the other 21 patients with normal herniography were asymptomatic via other treatment 3 months after herniography. Conclusions Herniography is useful to diagnose the occult or recurrent inguinal indirect hernias in patients with inguinal region pain of unclear causes.
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Objective To investigate the level of cell adhesion molecule sialyl-LeX expression in colorectal carcinoma and its relation with carcinogenesis, differentiation, metastasis and prognosis. Methods Sialyl-LeX expression and its optical density in colorectal carcinoma (n=90) and remote normal mucosa (n=30) were quantitatively studied with microwave-LSAB immunohistochemical method combined with image analysis technique. Fifty-three patients were followed up. Results The weaker staining in remote normal colorectal mucosa was observed in very limited parts of some deep crypts. Positive rate of sialyl-LeX expression was only 16.7%(5/30). The positive expression of sialyl-LeX was observed in 83 of 90 patients with colorectal carcinoma(92.2%). The apical cytoplasma of cancer tubules, the luminal contents, and the cytoplasma of the cancer cells were strongly stained. The mean integral optical density of sialyl-LeX positive cell in poorly differentiated adenocarcinoma was significantly higher than that in highly differentiated and mucinous ones (P<0.01). It was markedly higher in patients with positive lymphatic nodes than that in negative ones (P<0.01). With followed-up for longer than 5 years, it was much lower in the alive cases than that in the dead (P<0.01). Conclusion These findings indicate that changes of sialyl-LeX expression and its optical density is related to carcinogenesis, differentiation, invasion and metastasis of colorectal carcinoma. It may be a good predicter for the prognosis of patients with colorectal carcinoma.
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Objective To investigate the level of cell adhesion molecules LeX , sialyl-LeX, LeA and sialyl-LeA expression in colorectal carcinoma and its relation with carcinogenesis, differentiation, metastasis and prognosis.Methods The expression forms of LeX,sialyl-LeX,LeA and sialyl-LeA were studied by immunohistochemistry method with catalysis signal amplification (CSA)in 90 carcinomas and 30 normal mucosal specimens of colon and rectum, as well as in 51 metastatic lymph nodes.Fifty-three patients were followed up. Results Their antigens were expressed in normal mucosa distant from carcinoma lesions with the following frequencies: LeX, 93 3% (28/30); sialyl-LeX, 16 7% (5/30); LeA, 93 3% (28/30); and sialyl-LeA, 90 0% (27/30), whereas in colorectal carcinomas, the expression frequency rate were: LeX, 90 0% (81/90); sialyl-LeX, 94 4% (85/90); LeA, 78 9% (71/90); and sialyl-LeA, 80 0%(72/90). In metastatic lesion of lymphode LeX, sialyl-LeX, LeA and sialyl-LeA were expressed in 68 7% (35/51),96 1% (49/51),66 7% (34/51) and 66 7% (34/51), respectively.Conclusions The alteration of Lewis-related carbohydrate antigens in cancer cell membranes, including sialylation and/or aberrant glycosylation,these alteration may be related to metastatic behavior. These results suggested that sialyl-LeX may be used for estimating infiltration, metastasis and prognosis as a tumor-associated antigen in colorectal carcinoma.