ABSTRACT
<p><b>BACKGROUND</b>MiR-34a dysregulation has been implicated in tumorigenesis and progression of gastric cancer, but its role in prognosis of patients with gastric cancer remains unknown. The aim of this study was to investigate the expression and prognostic significance of miR-34a in gastric cancer patients after radical gastrectomy.</p><p><b>METHODS</b>Quantitative real-time polymerase chain reaction was performed to detect the expression of miR-34a in human gastric cancer cell lines and tissues in 76 patients with gastric adenocarcinoma from China. Results are assessed for association with clinical features and overall survival (OS) using Kaplan-Meier analysis. Prognostic values of miR-34a expression and clinical outcomes were evaluated by Cox regression analysis. A molecular prognostic stratification scheme incorporating miR-34a expression was determined using receiver operating characteristic analysis.</p><p><b>RESULTS</b>The results show that the expression level of miR-34a was decreased in human gastric cancer cell lines and tissues, and down-regulated expression of miR-34a was associated with Lauren classification (P = 0.034). Decreased miR-34a expression in gastric cancer tissues was positively correlated with poor OS of gastric cancer patients (P = 0.013). Further multivariate Cox regression analysis suggested that miR-34a expression was an independent prognostic indicator for gastric cancer (P = 0.027). Applying the prognostic value of miR-34a expression to tumor node metastasis (TNM) stage system showed a better prognostic value in patients with gastric cancer than miR-34a expression (P = 0.0435) or TNM stage (P = 0.0249) alone.</p><p><b>CONCLUSION</b>The results reinforce the critical role for the down-regulated miR-34a expression in gastric cancer and suggest that miR-34a could be a prognostic indicator for this disease.</p>
Subject(s)
Humans , Cell Line, Tumor , Gastrectomy , Gene Expression Regulation, Neoplastic , Genetics , In Vitro Techniques , Kaplan-Meier Estimate , MicroRNAs , Genetics , Multivariate Analysis , Prognosis , Real-Time Polymerase Chain Reaction , Stomach Neoplasms , Genetics , General SurgeryABSTRACT
Objective To study the solute clearance effect of t he new concentrated anticoagulation hemodialysate of citrate for hemodialysis in patients with high risk of bleeding.Methods During the period of 2002-06~2003-12,42 patients with h igh risk of bleeding were divided into two groups according to hemodialysis mann ers.Group Ⅱ were hemodialyzed with new citrate anticoagulation hemodialysate wi thout any other anticoagulant and group Ⅰ were hemodialyzed with bicarbonate he modialysate with dalteparin anticoagulation.The BUN and Cr levels were determine d before and after dialysis and Kt/V and URR were calculated.In addition,ACT and iCa 2+ were also measured at the artery and venous end.Results ACT was extended and iCa 2+ decreased significantl y at the venous end compared with that at the artery end in group Ⅱ (P0.05).Conclusion The solute clearance effect of the new concentrated anticoagulation hemodialysate of citrate for hemodialysis in patients with high risk of bleeding is excellent.