ABSTRACT
Objective To investigate the expression of CDKN1C in bone marrow in myelodysplastic syndromes and clinical significance of the detection of syndrome and secondary acute myeloid leukemia patients.Methods 125 patients with MDS/ AML were selected as the research object,and selected 20 cases of healthy people as healthy control group,and to investigate the mRNA and protein expression in CD34+ cells in bone marrow the expression of MDS/AML in patients and the survival rate of different expression of CDKN1C,to analyze the factors for survival rate of patients with MDS by Cox regression,and analyze the different treatment methods in patients with MDS/AML.Results The expression lcvcls of CDKN1C mRNA and protein of bone marrow CD34 + cells in the patients with MDS/AML were significantly higher than the healthy control group (t=5.324,7.326;P=0.002,0.000),and which was positive with BM count (r=2.014,P=0.004);the survival rate of CDKN1C high expression levels in patients with MDS/AML was significantly lower than that of the low expression of CDKN1C group and intermediate CDKN1C expression group (P＜0.05).Cox regression analysis showed that age,high BM count,cytogenetic risk difference and CDKN1C positive significantly affect the survival rate of patients with MDS/AML (95％CI=1.10～1.32,1.92～4.40,1.18～2.67,1.03～2.32,P=0.034～0.000).MDS/AML chemotherapy in CDKN1C positive group was significantly lower than the survival rate of patients with CDKN1C negative expression group (t=5.314,P=0.002).Conclusion The expression of CDKN1C in bone marrow of patients with MDS/AML was significantly increased,and the high expression of CDKN1CMDS/AML effect of chemotherapy on the survival rate of patients with MDS/AML.
ABSTRACT
The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor kappaB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0, 10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast differentiation and activation were monitored via tartrate-resistant acid phosphatase (TRAP) staining, filamentous-actin rings analysis, and a bone resorption assay. Furthermore, the expression osteoclast-related genes, such as TRAP and receptor activator for nuclear factor kappaB (RANK), that was influenced by OPG in RAW264.7 cells was examined using real-time polymerase chain reaction. In summary, findings from the present study suggested that M-CSF with RANKL can promote osteoclast differentiation and activation, and enhance the expression of TRAP and RANK mRNA in osteoclasts. In contrast, OPG inhibited these activities under serum-free conditions.