ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of substance P on cultured rat osteoclasts.</p><p><b>METHODS</b>Neurokinin-1 (NK1) receptor expression in osteoclasts was examined by immunohitochemical method, and changes of bone resorption activity caused by substance P and NK1 receptor antagonists were detected by pit formation assay.</p><p><b>RESULTS</b>Immunoreactivity for NK1 receptor was distributed in the cytoplasm of osteoclasts. The average of pit formation areas significantly increased with addition of substance P (10(-7)-10(-4) mol/L) (P < 0.05), but the number of pitformations did not change (P > 0.05). NK1 receptor antagonists inhibited the enhancement of the bone resorption by substance P addition.</p><p><b>CONCLUSION</b>The findings suggested that substance P may stimulate osteoclasts and result in bone resorption by the mediation of NK1 receptor.</p>
Subject(s)
Animals , Rats , Osteoclasts , Receptors, Neurokinin-1 , Substance PABSTRACT
<p><b>OBJECTIVE</b>To evaluate the outcome of the treatment of malocclusion with mandibular congenital missing teeth guided by Bolton index.</p><p><b>METHODS</b>26 class I and II cases (males 10, females 16) were chosen in this study. Guided by Bolton index, enamel stripping, denture and two types of tooth extraction were used in the treatment.</p><p><b>RESULTS</b>Normal Bolton index was achieved and the results of treatment were satisfactory.</p><p><b>CONCLUSIONS</b>Malocclusion with mandibular congenital missing teeth can be treated successfully guided by Bolton index.</p>