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Objective To investigate the incidence and risk factors of infection in neurosurgical patients in Wuxi people′s hospital,and to provide reference for the prevention of postoperative infection. Methods A total of 53 patients with postoperative infection were collected.The design method of one to three matching case-control study was used controlling age,sex and neurosurgical disease.The risk factors of infection were analyzed by retrospective analysis. Results Among 212 patients, 53 developed infections, the incidence rate was 25.0%(53/212). Respiratory tract infection was the main type of infection (60.4%,32/53).Patients with high blood glucose, high white blood cells, high hemoglobin, preoperative antibiotics in the infection group(t/χ2=3.486, 2.966, 2.196, 30.24, P<0.05). Patients in the infection group had emergency operation,long operation time,intra-operative blood loss,intra-operative blood transfusion(U/χ2=26.39,2.585,3.409,9.792,P<0.05).The infection group patients with postoperative use of antibiotics more than 2 kinds, long hospitalization, The difference between the two groups was statistically significant (U/χ2=85.53, 5.810,P<0.05). Further Logistic regression analysis showed that preoperative high blood sugar, intraoperative blood loss, operative duration and hospitalization time were the risk factors of nosocomial infection. Conclusion Risk factors for infection in department of neurosurgery are multiple and targeted interventions should be taken to reduce postoperative infection rate.
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10.3969/j.issn.2095-4344.2013.25.007
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<p><b>BACKGROUND</b>It has been proved that hypoxia is closely related to oncogenesis and development of tumor. The aim of this study is to observe the effect of dexamethasone on expression of hypoxia inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in lung tissues of hypoxic mice, and to investigate the relationship between hypoxia and angiogenesis and mechanism of dexamethasone.</p><p><b>METHODS</b>The Kunming mice were randomly divided into control group and three experimental groups (3-day hypoxia group, 6-day hypoxia group, and hypoxia+dexamethasone group). HIF-1α and VEGF protein expression was detected in lung tissues of mice by immunohistochemistry.</p><p><b>RESULTS</b>Expression of HIF-1α and VEGF significantly increased in hypoxia group compared with control group (P < 0.05). Compared with hypoxia group, expression of HIF-1α and VEGF decreased dramatically in hypoxia+dexamethasone group (P < 0.05). A positive correlation was found between the expression of HIF-1α and VEGF (r=0.730, P=0.007).</p><p><b>CONCLUSIONS</b>Hypoxia can increase the expression of VEGF and HIF-1α in lung tissues of mice. Dexamethasone can inhibit the expression of VEGF and HIF-1α of hypoxic mice and it may have anti-angiogenetic effect.</p>
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Objective To investigate the expression of hypoxia inducible factor-1?and VEGF in lung of mice during hypoxia and its relationship with angiogenesis.Methods The mice were divided into two group(hypoxia group and control group).The hypoxia group mice were placed in a hypoxia chamber with 10%,7%,or 5%O2 respectively for 3,6,9 days.Immuno- histochemical staining technique was used to examine HIF-1?,VEGF and MVD.The p rotein expression of HIF-1? and VEGF were observed by Western blot.Results HIF-1?proteins didn't express and VEGF proteins expressed weakly in control group mice;Compared with the controls,the expression of HIF-1?and VEGF increased dramadically.There were significant differences between the hypoxia groups and the control group;Positive relationships were found between the expression of HIF-1?,VEGF and MVD.Conclusion HIF-1?/VEGF pathway may play an important role in angiogenesis of hypoxia in the lung of mice.
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Objective To evaluate the safety of laparoscopic cholecystectomy's in aged patients in plateau region.Method From January 2005 to June 2006,a total of 64 local aged gallbladder disease patients were included,in which 32 cases received open cholecystectomy(OC)and 32 cases received laparoscopic cholecystectomy(LC).Postoperative cardiopulmonary complications were compared.Results In the LC group,1 case of acute pneumonedema,1 case of chronic right heart failure and 1 case of pulmonary infection occurred;and 3 cases of left heart failure accompanied with pneumonedema,2 cases of right heart failure and 5 cases of pulmonary infection occurred in the OC group.The incidence of cardiopulmonary complications of the LC group was significantly lower than that of the OC group(9% vs 31%,?2=4.730,P=0.030).Conclusions In the circumstances of high altitude,low atmospheric pressure and low oxygen partial pressure,LC is safer than OC for aged patients in regard to postoperative complications.