Simultaneous Determination of 5 Main Components in the Fruits and Root of Wild Acanthopanax senticosus by UPLC / 中国药房

OBJECTIVE:To establish a method for the 5 main components (original syringin,chlorogenic acid,eleutheroside E,isofraxidin and quercetin-3-rhamnoside) in the fruits and roots of wild Acanthopanax senticosus. METHODS:UPLC was per-formed on the column of Waters ACQUITY UPLC HSS T3 with mobile phase of acetonitrile-0.3% phosphoric acid (gradient elu-tion)at a flow rate of 0.2 ml/min. Detection wavelength was 300 nm,column temperature was 30 ℃,and injection volume was 10μl. RESULTS:The linear range was 24.56-184.2 μg/ml for syringin(r=0.9993),18.454-138.405 μg/ml for chlorogenic acid(r=0.9993),8.416-63.12 μg/ml for eleutheroside E (r=0.9997),3.286-24.645 μg/ml for isofraxidin (r=0.9993) and 2.522-18.915μg/ml for quercetin-3-rhamnoside(r=0.9998);RSDs of precision,stability and reproducibility tests were lower than 1%;recover-ies were 99.14%-100.50%(RSD=0.48%,n=6)for syringing in the fruits of A. senticosus、99.03%-100.45%(RSD=0.50%,n=6) for chlorogenic acid in the fruits of A. senticosus、99.22%-100.44%(RSD=0.44%,n=6)for eleutheroside E in the fruits of A. sen-ticosus、99.80%-100.80%(RDS=0.44%,n=6)for isofraxidin in the fruits of A. senticosus、99.76%-101.10%(RSD=0.51%,n=6) for quercetin-3-rhamnoside in the fruits of A. senticosus;99.21%-101.20%(RSD=0.73%,n=6)for syringing in the root of A. senti-cosus、99.81%-101.20%(RSD=0.52%,n=6)for chlorogenic acid in the root of A. senticosus、100.00%-101.50%(RSD=0.62%, n=6)for eleutheroside E in the root of A. senticosus、99.22%-100.40%(RSD=0.47%,n=6)for isofraxidin in the root of A. senti-cosus. CONCLUSIONS:The method is simple and stable with good reproducibility,and can be used for the simultaneous determi-nation of original syringin,chlorogenic acid,eleutheroside E,isofraxidin and quercetin-3-rhamnoside in the fruits and root of wild A. senticosus.

Content Determination of Rutin in Stems,Leaves,and Fruits of Sambucus williamsii Hance by HPLC / 医药导报

Objective To determine the content of rutin in fruits,stems,and leaves of Sambucus williamsii Hance,and provide an essential reference for the exploitation and utilization of the resources. Methods The content of rutin was analyzed on an Agilent ZORBAX Eclispse XDB C18 column(150 mmí4. 6 mm,5 μm). The mobile phase was methanol-0. 1%phosphoric acid (4555),the column temperature was set at 25 ℃,the flow rate was 0. 8 mL·min-1,and the detection wavelength was set at 360 nm. Results The linear range of rutin was 0. 105-0. 525 μg (r=0. 999 6),the average recovery was 97. 86%,and RSD was 0. 49%. The content of rutin in stems, leaves, and fruits of Sambucus williamsii Hance was 0. 171, 0. 522, and 0. 165 mg·g-1 ,respectively. Conclusion The stems,leaves and fruits of Sambucus williamsii Hance all contain rutin,but at a significant different content level. The leaves contain the highest level of rutin.