ABSTRACT
Aim To observe the damages induced by hydrogen peroxide in cultured bovine cerebral microvascular endothelial cells (BCMEC) and evaluate the protective effects of hydroxyethylpuerarin on hydrogen peroxide-injured BCMEC. Methods BCMEC were cultured and transferred into modified Eagle medium (MEM). The viability of cells was detected by MTT assay. Cell injury was determined by lactate dehydrogenase (LDH) activity in the extracellular medium. Flow cytometry was employed to observe the occurrence of apoptosis. Morphologic changes of cells were visualized under phase contrast and electron occurrence of apoptosis. Hydroxyethylpuerarin was shown to increase the survival rate and decrease the activity of LDH of BCMEC damaged by hydrogen peroxide. Hydroxyethylpuerarin was also found to protect BCMEC against apoptosis induced by hydrogen peroxide. Conclusion Hydrogen peroxide induces BCMEC injury either by apoptosis or through necrosis. Hydroxyethylpuerarin protects BCMEC against hydrogen peroxide-induced injury in a concentration-dependent manner. Its antioxidant effects might be involved as the mechanism protection.
ABSTRACT
The effects of Proplylene glucol mannurate sulfate (PGMS) on mice's immunological function have been studied with immunological techniques. When given orally,PGMS could enhance the weight of the mice's immunological organs and the speed of carbonparticle elimination in mice,the phagocytic rate and index of the macrophagocytes in the mice's abdominal cavity were enhanced, the percentage of ANAE" lymphocytes were increased, the production of hemolysin in mice peripheral blood were promoted. The study suggests that PGMS improves both the specific and nonspecific immunological function in mice.