ABSTRACT
Objective To study the molecular mechanism about injurious effect of morphine dependence on the construction and function of hippocampal neurons. Method Mice were given (sc) increasing doses of morphine to form morphine-dependence model ,and the DNA and RNA changes stained with acridine orange (AO) fluorescence probe technique were investigated in hippocampal neurons of morphine-dependence group,naloxone-precipitated withdrawal group in morphine-dependence mice and control group. Results Compared with control group , the staining changes of DNA,RNA decreased obviously in hippocampal neurons of both morphine-dependence group and naloxone- precipitated withdrawal group in morphine-dependence mice,especially the later. Conclusion Both morphine-dependence and naloxone-precipitated withdrawal in morphine-dependence mice injured nucleic acid metabolism in hippocampal neurons ,especially the later.Those changes may be some reasons of decreased brain function ,especially in learning and memory deficits.
ABSTRACT
Objective:To study the difference of c-fos expression of neurons in subareas of hippocampus of morphine dependent mice.Methods:Mice were given (sc) increasing doses of morphine to form morphine dependent models and withdrawal syndromes were precipitated by naloxone. The intensity of withdrawal syndromes was evaluted accoding to indices ,such as the number of jumping ,the weight loss,et al .The expression of Fos positive neurons in subareas of CA1, dentate gyrus and CA3 of hippocampus of morphine dependent group was observed by immunohistochemistry assay. Results:The number of Fos positive cells in subareas of CA1 and dentate gyrus of morphine dependent group was much higher than that of the normal control group(P
ABSTRACT
Objective:To compare the interaction of withdrawal behaviors and correlated indices of two morphine dependent models of mice.Method: Mice were given morphine with gradual increasing dose to establish two models of morphine dependence with different dosage sequence, i.e. model 1 for 7 days, model 2 for 5 days. Withdrawal syndrome was precipitated by naloxone and recorded. The weight of heart, liver, lung, kidneys, adrenal glands, testicles of the mice were compared.Result:Both methods (7 days way and 5 days way) successfully established morphine dependent models in mice. In withdrawal, the mice of 7 days model had more average times of jump and “wet-dog” shaking than the mice of 5 days model had, they had also more obvious upper eyelid dropping and anterior claw tremble. The weights of heart, kidneys, testicles of the mice of 7 days model were significantly lower than the counterparts of the mice of 5 days model. Conclusion:Both 7 days way and 5 days way can establish morphine dependent model of mice, the former has advantage of more indexes applicable while the later has advantage of less dose of morphine and short time needed.