The changes of specific binding sites of glucocorticoid after scalding in rats / 中国病理生理杂志

The binding capacity (R_o) and apparant dissociation constant (K_d) of glucocrticoid binding sites in hepatic cytosol of normal and scalded rats were measured by pseudoscatchard analysis. Compared with control group, the levels of Ro of glucocorticoid receptor was significantly decreased 1 h after scalding, partialy recovered at 12h after scalding and fully recovered at 24 h after scalding. The levels of low-affinity glucocorticoid binding sites were also decreased 12 h after scalding, but had no statistical significance. The possible significance of these results has been discussed.

Study on Gucocorticoid Receptor in Rats with Traumatic Brain Edema / 第二军医大学学报

The high-affinity glucocorticoid binding sites (HAGS) and the low-affinity glucocorticoid binding sites (LAGS) with steroid specificity were demostrated in cerebral cytosol of rats by using the radioligand binding assay. The Kd of HAGS and LAGS were (178?0.71)?l0-8mol/L and (2.12?1.06)?10-6mol/L respecitively as estimated by Scatchard and Pseudoscatchard analysis. Glucocorticoid receptors (GR) in the traumatized(left) hemisphere cytosol were decreased more significantly than those in both the control (right) hemisphere cytosol at 6h postinjury and normal brain tissue (P

The Basic Characteristics of Low-affinity Glucocorticoid Binding Sites / 第二军医大学学报

Scatchard, pseudoscatchard and competitive analyses showed that there were two glucocorticoid (GC) binding sites in rat hepatic cytosol: high-affinity glucocorticoid binding sites (HAGS) and low-affinity glucocorticoid binding sites (LAGS). Pseudoscatchard analysis showed the dissociation constant (Kd) of LAGS was 4.1+1.4umol/L (n=4). The inactivation of LAGS in vitro was slower" than that of HAGS. LAGS and HAGS could not be separated by DEAE-cellulose chfomatography. HAGS were saturable and had steroid specificity for glucocorticoid just as the classic glucocorticoid receptor. Triamcinolone acetonide, RU486 and RU26988 competed equally well to LAGS while aldosterone, progesterone, estradiol and testosterone did not compete with LAGS. These results suggest that LAGS have steroid specificity for glucocorticoids. The biological actions of LAGS await fur ther study.