ABSTRACT
AIM:To study the chemical constituents of Lysimachia clethroide Duby. METHODS:Chromatographic techniques,including MS and NMR,were used to isolate and purify the constituents based on column chromatography separation on silica gel,Sephadex LH-20 and Semipreparative RP-HPLC. RESULTS:Eleven flavonoids were obtained and elucidated as: kaempferol (1),kaempferol 3-O-?-D-glucopyranoside (2),kaempferol 3-O-?-D-galactoside (3),kaempferol 3-O-?-D-(6″-p-coumaroyl)-glucopyranoside (4),quercetin (5),Isorhamnetin (6) ,quercetin 3-O-?-D-galactoside (7),quercetin 3′-methoxy-3-O-?-D-galactoside (8),quercetin 3-O-?-D-(6″-p-coumaroyl)-galactoside (9),4′-methoxy-5,6-dihydroxyisoflavone-7-O-?-D-glucopyranoside (10),7-O-glucosyl liquiritigenin (11). CONCLUSION: Compounds 3,6 were isolated from this plant and compounds 8-11 were isolated from this genus for the first time.
ABSTRACT
AIM: To evaluate the effect of microfiltrating the extract of Sophora japonica L. by inorganic ceramic membrane. METHODS: The extract of Sophora japonica L. was processed by inorganic ceramic membrane, and the whole solid, effective ingredinent and flux of mebrane were examined. RESULTS: The extract of Sophora japonica L. became clear after microfiltration. The decreasing rate of the whole solid was 27.88%, the metastasis rate of the effective ingredient was 78.26, and the increasing rate of the effective ingredient was 8.48%. CONCLUSION: The microfiltration of inorganic ceramic membrane can improve the qualitry of extract of Sophora japonica L. refinery effect.