ABSTRACT
Objective To study the Chlamydia trachomatis(CT)and Ureaplasma urealyticum (UU)infection in Guangzhou area, and analyze the consistency of simultaneous amplification and testing (SAT)and conventional methods(CT was detected by latex immunochromatography, UU was detected by liquid culture method).Methods A total of 12 120 samples of urogenital secretions or urine samples were collected from Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University from January 2015 to December 2016.CT-RNA and UU-RNA were detected by the SAT technique, a part of samples were tested by conventional methods at the same time.The positive rates of CT and UU by SAT and the conventional methods between different gender and age groups were analyzed by χ2test, the consistencies between different detection methods were analyzed by Kappa test.Results The positive rate of CT was 4.05%(356/8 781), UU 33.69%(1 125/3 339)in Guangzhou from 2015 to 2016.The positive rate of UU was significantly higher than that of CT(χ2=1 981,P<0.01).Of 145 specimens for CT test,the coincidence rate between SAT and latex immunochromatographic method was 96.55%(140/145), which showed good consistency(Kappa=0.65).Of 186 specimens for UU test,the coincidence rate of the results between the SAT method and liquid culture was 92.47%(172/186),which showed strong consistency(Kappa=0.81). Conclusions The positive rate of UU was significantly higher than that of CT in Guangzhou.The SAT method and conventional methods to detect CT and UU show high consistency, which can provide the evidence for clinical diagnosis of CT and UU infection.
ABSTRACT
Objective: to analyse the effects of combined acupuncture anesthesia on surgical dosage and serum interleukin-4 [IL-4], interleukin-10 [IL-10] of pneumonectomy patients
Study Design: a randomised controlled trial [RCT]
Place and Duration of Study: lanzhou University Second Hospital, Lanzhou, China, from January 2016 to November 2017
Methodology: a total of 110 patients undergoing pneumonectomy were randomly divided into group A and group B, with 55 cases in each group. Group A was treated with combined 2Hz acupuncture anesthesia, while group B was treated with combined 2Hz/100Hz acupuncture anesthesia. The additional propofol, fentanyl dosage, and changes in heart rate and systolic blood pressure 5 min before and during extubation were compared between the two groups. The serum IL-4 and IL-10 levels were measured 10 minutes after skin incision and 24 hours after surgery using ELISA. Pain was rated by visual analogue scale [VAS] at 24 hours after surgery
Results: there was no statistically significant difference between group A and group B in the intraoperative additional propofol and fentanyl dosages [p=0.541 and 0.719, respectively]. There is no statistical difference in heart rate and systolic blood pressure between group A and group B at 5 minutes before and during extubation [p=0.151, 0.660 and 0.734, 0.528, respectively]. There is no statistical difference between group A and group B in serum IL-4 and IL-10 levels 10 minutes after surgical incision [p=0.916 and 0.841, respectively]. However, serum IL-4 and IL-2 levels in group A are statistically lower than that of group B at 24 hours after surgery [all p<0.001]. The VAS score at 24 hours after surgery in group A was 2.44 +/- 0.71 points, which was significantly lower than that of group B [3.82 +/- 0.77 points, p<0.001]
Conclusion: combined 2 Hz, 2 Hz/100 Hz acupuncture anesthesia need few anesthetic dosages of propofol and fentanyl to stabilise the patient's blood pressure and heart rate when intubated under general anesthesia; but combined 2 Hz acupuncture anesthesia can reduce IL-4 and IL-10 levels during surgery stress to a greater extent than the latter, and can effectively lower patients' serum IL-4, IL-10 expression after surgery
ABSTRACT
Objective:To detect the contents of hyaluronic acid(HA),Laminin(LN) and probe their role in the pathologic course of chronic renal failure(CRF).Methods:The serum HA and LN from 37 patients with CRF and 40 healthy adults were measured by RIA.T test was used in data(x?s) analysis.Results:The HA and LN in CRF serum evidently increased than those of normal controls,respectively(P