ABSTRACT
In order to improve the training quality of non anesthesiology professional physicians in anesthesiology department, the anesthesia department of Cancer Hospital affiliated to Harbin Medical Uni-versity Anesthesiology summed up the problems existing in the rotation process of anesthesiology depart-ment in the past, and explored the aesthesia teaching of the standardized doctors. We also improved the teachers' and students' awareness of the importance of anesthesiology learning, formulated standardized training programs, stimulated the interest of residents and teachers, strengthened teachers' teaching ability and teaching consciousness, Through the use of special lectures, case teaching, problem based teaching, we constructed diversified teaching to enhance the students' mastery of knowledge and skills in anesthesiology, and added purposeful examination at the end of rotation. As a result, the quality of anesthesiology rotation of non-anesthesia specialty residents was improved.
ABSTRACT
Objective The objective of this study was to investigate the effect of UHRF1 on the prolifer-ation and metastasis of breast cancer MDA-MB-231 cells and its mechanism. Methods The effect of silen-cing UHRF1 gene on the viability of MDA-MB-231 cells was detected by MTT assay. Colony formation assay was performed to analyze the effect of silencing UHRF1 on cell survival of MDA-MB-231 cells. The effect of silencing UHRF1 on the apoptosis of MDA-MB-231 cells was detected by acridine orange-ethidium bromide ( AO / EB) . Caspase-3 activity kit was used to detect the expression of caspase-3 in MDA-MB-231 cells. The expressions of Bcl-2,Bax,Bad,p-Bad,XIAP,p53,p21Cip1/Waf and p16INK4a were detectedby Western blot. The abilities of invasion and migration of MDA-MB-231 cells silenced by UHRF1were examined by Transwell and Wound healing assays,respectively. Results Silencing UHRF1 significantlydecreased the viability of MDA-MB-231 cells. Silencing UHRF1 decreased colony formation in MDA-MB-231 cells. Depletion of UHRF1 resulted in apoptosis inducedin MDA-MB-231 cells,showing nuclear morphological changes by AO/EB stai-ning and increasing caspase -3 activity. After knockdown of UHRF1,the expression of Bad,XIAP,Bax,p53, p21Cip1/Waf1 and p16INK4a was up-regulatedand down-regulated the expression of p-Bad and Bcl-2 in MDA-MB-231 cells. Transwell and wound healing assays demonstrated that silencing UHRF1 could decrease metasta-sisin MDA-MB-231 cells. Conclusion Silencing UHRF1 can inhibit the viability and survival of MDA-MB-231 cells,and inhibit the invasion and migration of MDA - MB -231 cells regulated by p53/p21Cip1/Waf1/p16INK4asignalings.