ABSTRACT
OBJECTIVE: To develop a RP-HPLC method for simultaneous determination of hesperidin, nitidine chloride, ehelerythrine, and toddalolactone in Toddalia asiatica(L)Lam. Methods: The RP-HPLC system consisted of a Diamonsil C18 column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-water solution containing 75% glacial acetic acid. UV detector was used, and the detection wave length was 290 nm. Gradient elution was adopted at a flow rate of 1.0 mL · min-1 and the column temperature was 30°C. For different constituents, external standard method was used with the peak area at 290 nm as the quantitative index. RESULTS: The liner ranges of hesperidin, nitidine chloride, ehelerythrine and toddalolactone were 0.4874-2.4372 μg(r= 0.9997), 0.0303-0.1515 μg(r=0.9992), 0.0623-0.3117 μg(r=0.9993), and 0.0249-0.1246 μg(r=0.9999), respectively. The average recoveries(n=6) were 100.76%(RSD 2.75%), 97.98%(RSD 1.25%), 100.07%(RSD 3.24%), and 100.10% (RSD 3.83%), respectively. CONCLUSION: The method is accurate, simple, rapid, and reproducible for the determination of hesperidin, nitidine chloride, ehelerythrine, and toddalolactone in Toddalia asiatica(L)Lam. The determination results can be used as a reference for the rational medication, quality control, and further study of Toddalia asiatica(L.)Lam.