ABSTRACT
Proton nuclear magnetic resonance(~1H-NMR) is used to investigate the effect of Renshenjian Decoction on serum and urine metabolism of type 2 diabetic rats with insulin resistance induced by high-sugar and high-fat diet combined with low-dose streptozotocin(STZ). After the successful establishment of the insulin resistance model of type 2 diabetes, administration for 35 days, the serum and urine of rats were taken. Once the ~1H-NMR data have been collected and processed, PCA and OPLS-DA were used to analyze them. The results show that: compared with the blank group, the contents of methionine, taurine, α-glucose and β-glucose in the serum of the model group increased significantly(P<0.001), while the contents of 3-hydroxybutyric acid, lactic acid and unsaturated fatty acids decreased significantly(P<0.01). In the model group, the contents of trimethylamine oxide, glycine, α-glucose, β-glucose, taurine and phosphocholine in urine increased significantly(P<0.05), while the contents of creatine, lactic acid, acetic acid and citric acid decreased significantly(P<0.05). Compared with the model group, the contents of 3-hydroxybutyric acid and unsaturated fatty acids in serum of rats in the treatment group increased significantly(P<0.05), while the contents of taurine, α-glucose and β-glucose decreased significantly(P<0.01). In the treatment group, the contents of lactic acid, taurine and creatine in urine increased significantly(P<0.05), while the contents of trimethylamine oxide, glycine, α-glucose, β-glucose and phosphocholine decreased significantly(P<0.01). The results show that Renshenjian Decoction can regulate metabolic disorder and promote the metabolic phenotype to return to the normal range. It displayed therapeutic effect on type 2 diabetic rats with insulin resistance and provided a certain scientific basis for the biological basic research of Renshenjian Decoction by improving insulin resistance in diabetes mellitus.
Subject(s)
Animals , Rats , Blood Glucose , Diabetes Mellitus, Experimental , Diabetes Mellitus, Type 2 , Metabolomics , Proton Magnetic Resonance Spectroscopy , Rats, Sprague-DawleyABSTRACT
Objective To clarify the toxicity reducing mechanism of vinegar-processed Kansui by comparing the plasma metabonomic profiles induced by Kansui and vinegar-process Kansui treatments. Methods Totally 30 SD rats were divided into five groups: controls, 7. 875 g/kg Kansui, 15. 75 g/kg Kansui, 7. 875 g/kg vinegar-processed Kansui and 15. 75 g/kg vinegar- processed Kansui. The treatments lasted for 2 weeks. The plasma sampleswere obtained before, immediately after and 7 days after withdrawal of drugs; the 1 HNMR spectra of each sample were obtained and analyzed by orthogonal partial least-squares- discriminant analysis (OPLS-DA) method. Results Compared with Kansui groups, vinegar-processed Kansui increased plasma lipids, 3-hydrobutyrate and glucose, and decreased the levels of lactate and creatine/creatinine; furthermore, the spectra profiles of vinegar-processed Kansui groups were closer to those of the control group. Seven days after drug withdrawal, the rats of vinegar-processed Kansui groups recovered better than those in Kansui groups. Conclusion Vinegar-processed Kansui has toxicity-reducing effect; it can regulate the metabolism of energy and fat, and reduce the toxicity to the liver and kidney. Plasma metabonomics study based on 1 HNMR spectroscopy and multivariate analysis can be used to explore the toxicity- reducing mechanism of vinegar-processed Kansui.
ABSTRACT
Objective To characterize the metabolite composition (i. e., metabonome) of the serum samples from chronic hepatitis B patients by using nuclear magnetic resonance 1H (1H NMR) spectroscopy combined with principal component analysis (PCA). Methods The 1H NMR sepctra were obtained for the serum samples from 8 healthy controls and 20 chronic hepatitis B patients, and the spectra data were analyzed by PCA to demonstrate the metabonomic changes. Results We found that there were evident differences in metabonomics between the healthy controls and chronic hepatitis B patients. The levels of lipids and glucose were significantly increased in the chronic hepatitis B patient and the levels of lactate, alanine, valine, glutamine and phosphocholine/choline were significantly decreased compared with the healthy controls (P<0. 05). Conclusion Our results indicate that the metabonomes of serum from chronic hepatitis B patients are different from those of the healthy controls. Serum 1H NMR sepctroscopy combined with PCA may offer reliable biochemistry evidence for diagnosis of chronic hepatitis B at molecular level.