ABSTRACT
Objective: To establish an HPLC method for the content determination of salvianolic acid B, fumalic acid, hydroxysafflor yellow A, gallic acid, and caffeic acid in Danxiong Tongmai Granule. Methods: The determination was carried out by HPLC, using Thermo ODS-2 Hypersil (250 mm×4.6 mm, 5 μm) chromatographic column. The mobile phase was 0.1% acetic acid solution-methyl alcohol, gradient elution, and UV detection wavelength was set at 286 nm. The column temperature was set at 30 ℃. The injection volume was 10 μL. Results: Under the above chromatographic conditions, there was a good linearity between the absorption peak area and the concentration in the ranges of 3.18-50.88 μg/mL (r=0.9990) for salvianolic acid B, 2.00-32.00 μg/mL (r=0.9995) for fumalic acid, 6.00-96.00 (r=0.9996) for hydroxysafflor yellow A, 0.60-9.60 μg/mL (r=0.9994) for gallic acid, and 1.00-16.00 μg/mL (r=0.9995) for caffeic acid, respectively. Conclusion: This method is simple, fast, accurate, reliable, and reproducible, and it is proper for the content determination of salvianolic acid B, fumalic acid, hydroxysafflor yellow A, gallic acid, and caffeic acid in Danxiong Tongmai Granule at the same time.
ABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents in the heartwood of Taxus cuspidata.</p><p><b>METHOD</b>Silica gel column chromatography, preparative HPLC and preparative TLC were used to isolate and purify the chemical constituents; 1H- and 13C-NMR spectroscopic methods were used for structural identification.</p><p><b>RESULT</b>Ten compounds, taxinine (1), taxusin (2), beta-sitosterol (3), 1 beta-hydroxybaccatin I (4), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl) butanoyloxy-4 (20), 11-taxadiene (5), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl-3'-hydroxy-butanoyloxyl-4 (20), 11-taxadiene (yunnanxane) (6), 9alpha, 10beta, 13alpha-triacetoxy-5alpha-cinnamoyltaxa-4 (20), 11-diene (7), 2-deacetoxytaxinine J (8), taxezopidine G (9), 2alpha, 7beta, 9alpha, 10beta, 13alpha-pentaacetoxyl-taxa-4 (20), 11-dien-5-ol (5-decinnamoyltaxinine J) (10), were isolated and identified from the heartwood of T. cuspidata.</p><p><b>CONCLUSION</b>Three taxanes, 1 beta-hydroxybaccatin I (4), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl-3'-hydroxy-butanoyloxy)-4 (20), 11-taxadiene (yunnanxane) (6), and 2alpha, 7beta, 9alpha, 10beta, 13alpha-pentaacetoxyltaxa-4 (20) , 11-dien-5-ol (10), were obtained from this plant for the first time.</p>
Subject(s)
Diterpenes , Chemistry , Heterocyclic Compounds, 4 or More Rings , Plant Stems , Chemistry , Plants, Medicinal , Chemistry , Taxoids , Chemistry , Taxus , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between redundant prepuce and premature ejaculation.</p><p><b>METHODS</b>Fifty-two cases suffering from premature ejaculation and redundant prepuce were treated with circumcision. They were asked to fill the investigating questionnaire about the changes of ejaculatory latent period, patients' and their wives' satisfaction with sexual life before and after the treatment.</p><p><b>RESULTS</b>During 12 months after circumcision, 28 cases were cured and 11 cases were efficacious. The curative rate was 54.9% and effective rate was 76.5%. Twelve cases with no responding continued to be treated with routine methods, such as psychotherapy, daub narcotic to glans of penis and taking medicine to treat chronic prostatitis, which were used before circumcision but still no effects. During 18 months after circumcision, 4 cases were cured and 5 cases were efficacious.</p><p><b>CONCLUSION</b>Redundant prepuce had direct or indirect relationship with premature ejaculation. The circumcision is one of the effective methods to treat premature ejaculation.</p>