Research progress on chemical composition and pharmacological effects of Gardenia jasminoides and predictive analysis on quality marker (Q-marker) / 中草药

Gardenia jasminoides is a traditional Chinese herbal medicine, which is also the first batch of being used for both medicine and food issued by Ministry of Health of China. In recent years, G. jasminoides has been applied widely in medicine and health food, so the quality evaluation of G. jasminoides has become a key problem urgently needed to be solved in this industry. Based on the review of its chemical composition and pharmacological effects, combined with the definition of Q-marker, this study processed predictive analysis on Q-marker of G. jasminoides from several aspects at chemical composition, new clinical use, measurable composition, traditional medicine properties and efficacy, plasma composition, and storage time, which provides the basis for quality evaluation of G. jasminoides.

Real-time effect of adipose stem cells on fibrogenesis of dermal fibroblast co-stimulated by transforming growth factor-β1 / 上海交通大学学报（医学版）

Objective • To mainly explore the real-time effect of adipose stem cells (ASCs) on the fibrogenesis of dermal fibroblasts co-stimulated by transforming growth factor-β1 (TGF-β1), and further clarify the possible pathway and mechanism of ASCs in regulating wound repair. Methods • By using two different real-time culture systems including Transwell system and contact co-culture system, events associated with fibrogenesis including the changes of fibroblast cell number or expression of collagen types and III detected by immunofluorescence or Western blotting in dermal fibroblasts at 72 h with/without the stimulation of transforming growth factor-β1 (TGF-β1) and/or ASCs were studied. Results • In Transwell system, the cell number of fibroblasts was significantly decreased under the stimulation of ASCs and TGF-β1, compared with TGF-β1 only group (P=0.035). In contact co-culture system, under the stimulation of TGF-β1, the numbers of fluorescence labeling fibroblasts in group with ASCs as basal cells were decreased, compared with group with fibroblast as basal cells (P=0.000). In terms of the collagen expression, in Transwell system, the amounts of collagen secretion from fibroblasts within the upper chamber were increased dramatically when fibroblasts were being co-cultured with ASCs (P=0.000). In contact co-culture system, under the stimulation of TGF-β1, the amounts of collagen secretion in the supernatant of cell culture in the group with ASCs as basal cells were increased, compared with the group with fibroblast as basal cells (P=0.000). Conclusion • ASCs may have an effect on fibrogenesis of dermal fibroblasts co-stimulated by TGF-β1 through a paracrine and direct contact way. It not only increases collagen production and secretion, but also inhibits fibroblasts over-proliferation.

Protective effect of anti-hypoxia shenggui tablet on cerebral ischemia injure / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To investigate the protective effects of Shengui tablet (Chinese Traditional Medicine) on experimental cerebral ischemia by acute cerebral ischemia hypoxia in mice and bilateral ligation of the carotid artery in rats.</p><p><b>METHODS</b>In the acute cerebral ischemia hypoxia model, the mice were randomly divided into control group, low-, middle- and high-dose (0.16, 0.33 and 1.00 g/kg) groups of Shengui tablet, after oral treatment for 30 d, gasping time of isolated heads of mice were observed. In bilateral ligation of the carotid artery cerebral ischemia model, the rats were randomly divided into control group, model group and low-, middle-, high-dose (0.072, 0.149 and 0.450 g/kg) groups of Shengui tablet. After oral treatment for 7 d, the cerebral index, superoxide dismutase (SOD) activity and the content of malondialdehyde (MDA) were measured.</p><p><b>RESULTS</b>Compared with the control model, Shengui tablet middle- and high-dose could significantly prolong gasping time of isolate heads of mice. Compared with model group, Shengui tablet low-, middle- and high-dose could significantly decrease the cerebral index and enhance SOD activity in brain tissue; only high-dose could reduce the content of MDA.</p><p><b>CONCLUSION</b>Shengui tablet has significant protective effect on the cerebral ischemia.</p>

Protective effects of SUR2B/Kir6.1 potassium channel opener natakalim against RAVECs injuries induced by hypoxia / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To investigate the protective effects of natakalim against rat aortic vascular endothelial cells (RAVECs) injuries induced by hypoxia and its mechanisms.</p><p><b>METHODS</b>Selecting RAVECs as a cell model injured by hypoxia, these RAVECs were divided into 5 groups: i.e. control group, hypoxia group, natakalim low, medium and high group. The cell survival rate was determined by MTT assay, con was measured using Griess Assay, RT-PCR was used to examine t he expression of intercellular adhesion molecule-1 (ICAM-1), vascular endothelial growth factor (VEGF), endothelin-1 (ET-1) mRNA in RAVEC.</p><p><b>RESULTS</b>Natakalim could reverse hypoxia-induced changes in endothelial cell function, including increased endothelial cell survival rate and level of NO concentration, significantly inhibited the hypoxia-induced endothelial ICAM-1, ET-1, VEGF mRNA expression levels increased.</p><p><b>CONCLUSION</b>Natakalim have protective effects on hypoxia-induced changes in endothelial cell function, increasing of permeation, excess expression of cell adhesion molecules.</p>

Effect of casein and protamine on the enzymatic degradation and the orally hypoglycemic action of insulin / 药学学报

<p><b>AIM</b>To study the protection of casein and protamine against degradation of insulin (INS) by proteolysis enzymes and the effect of these two kinds of protein on the hypoglycemic action of INS solution and enteric-microspheres after administrated orally to rats.</p><p><b>METHODS</b>HPLC was used to determine the remained INS in the solution of alpha-chymotrypsin and trypsin with or without casein or protamine; INS solution and enteric-microspheres were prepared and adiministrated orally to rats together with the absorption enhancer sodium N-[8-(2-hydroxybenzoyl) amino] caprylate (SNAC). At the same time, casein or protamine or both of these two kinds of protein were administrated together in order to study their influence on the hypoglycemic effect of INS and microspheres.</p><p><b>RESULTS</b>Casein had a good protection against degradation of INS by alpha-chymotrypsin, but protamine had no protection effect. However, the degradation of INS by trypsin is concerned, the protection effect of protamine on INS was better that of casein. Both of protamine and casein can increase the hypoglycemic effect of INS solution and enteric-microspheres. Co-administrated these two kinds of protein had a better effect. In addition, co-administrated with SNAC, casein and protamine, INS enteric-microspheres had a longer and more potent hypoglycemic effect than that of the solution.</p><p><b>CONCLUSION</b>Casein and protamine can increase the stability of INS in the intestinal fluid by the mechanism of competition and combine with proteolysis enzymes, which will benefit to INS oral administration.</p>